Curated Information
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Home > Curated Information Page > PubMed Id: 14500679
Zhan Q, et al. (2003) p21-activated kinase 2 in neutrophils can be regulated by phosphorylation at multiple sites and by a variety of protein phosphatases. J Immunol 171, 3785-93 14500679
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S144-p - PAK1 (mouse)
Modsite: SNSKKYMsFTDKSAE SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S144‑p, PAK1 (mouse): S144‑p, PAK1 (rat): S144‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S199-p - PAK1 (mouse)
Modsite: PRPEHTKsVYTRsVI SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S199‑p, PAK1 (mouse): S199‑p, PAK1 (rat): S198‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S204-p - PAK1 (mouse)
Modsite: TKsVYTRsVIEPLPV SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S204‑p, PAK1 (mouse): S204‑p, PAK1 (rat): S203‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

T423-p - PAK1 (mouse)
Modsite: PEQSKRstMVGtPYW SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): T423‑p, PAK1 (mouse): T423‑p, PAK1 (rat): T422‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S20-p - PAK2 (mouse)
Modsite: APPVRMSstIFSTGG SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): S20‑p, PAK2 (mouse): S20‑p, PAK2 (rat): S20‑p, PAK2 (rabbit): S20‑p, PAK2 (cow): S20‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig

S141-p - PAK2 (mouse)
Modsite: TVkQKyLsFtPPEKD SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): S141‑p, PAK2 (mouse): S141‑p, PAK2 (rat): S141‑p, PAK2 (rabbit): S141‑p, PAK2 (cow): S141‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig

S192-p - PAK2 (mouse)
Modsite: PRPDHTKsIYtRsVI SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): S192‑p, PAK2 (mouse): S192‑p, PAK2 (rat): S192‑p, PAK2 (rabbit): S192‑p, PAK2 (cow): S192‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

S197-p - PAK2 (mouse)
Modsite: TKsIYtRsVIDPIPA SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): S197‑p, PAK2 (mouse): S197‑p, PAK2 (rat): S197‑p, PAK2 (rabbit): S197‑p, PAK2 (cow): S197‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase

T402-p - PAK2 (mouse)
Modsite: PEQSKRstMVGtPYW SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): T402‑p, PAK2 (mouse): T402‑p, PAK2 (rat): T402‑p, PAK2 (rabbit): T402‑p, PAK2 (cow): T402‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neutrophil
Cellular systems studied:  primary cells
Species studied:  guinea pig
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CB (mouse) pharmacological inhibitor of upstream enzyme
PHOSPHATASE PPP2CA (mouse) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fMLP increase
okadaic_acid fMLP augment treatment-induced increase
ascomycin fMLP augment treatment-induced increase
ciclosporin, fMLP fMLP augment treatment-induced increase
FK506 fMLP augment treatment-induced increase

S154-p - PAK3 (mouse)
Modsite: VNNQKYMsFTSGDKs SwissProt Entrez-Gene
Orthologous residues
PAK3 (human): S154‑p, PAK3 iso3 (human): S175‑p, PAK3 (mouse): S154‑p, PAK3 (rat): S139‑p

T436-p - PAK3 (mouse)
Modsite: PEQSKRstMVGtPYW SwissProt Entrez-Gene
Orthologous residues
PAK3 (human): T436‑p, PAK3 iso3 (human): T457‑p, PAK3 (mouse): T436‑p, PAK3 (rat): T421‑p