Lee S, et al. (2016) Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis. Neuron 91, 41-55 27321923

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S359-p - HIPK2 (human) ▲

Modsite: HVSkAVCstyLQsRY SwissProt Entrez-Gene Orthologous residues HIPK2 (human): S359‑p, HIPK2 (mouse): S359‑p, HIPK2 (rat): S359‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), brain Cellular systems studied:  cell lines, tissue Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes tunicamycin increase ASK1 (human) increase siRNA decrease Downstream Regulation Effect of modification (function):  enzymatic activity, induced, molecular association, regulation Effect of modification (process):  apoptosis, induced, signaling pathway regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ASK1 (human) Induces co-immunoprecipitation JNK2 (human) Induces co-immunoprecipitation Comments:  HIPK2 and JNK activation Associated Diseases Diseases Alterations Comments ALS increased

T360-p - HIPK2 (human) ▲

Modsite: VSkAVCstyLQsRYy SwissProt Entrez-Gene Orthologous residues HIPK2 (human): T360‑p, HIPK2 (mouse): T360‑p, HIPK2 (rat): T360‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), brain Cellular systems studied:  cell lines, tissue Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes tunicamycin increase ASK1 (human) increase siRNA decrease Downstream Regulation Effect of modification (function):  enzymatic activity, induced, molecular association, regulation Effect of modification (process):  apoptosis, induced, signaling pathway regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ASK1 (human) Induces co-immunoprecipitation JNK2 (human) Induces co-immunoprecipitation Comments:  HIPK2 and JNK activation Associated Diseases Diseases Alterations Comments ALS increased

Y361-p - HIPK2 (human) ▲

Modsite: SkAVCstyLQsRYyR SwissProt Entrez-Gene Orthologous residues HIPK2 (human): Y361‑p, HIPK2 (mouse): Y361‑p, HIPK2 (rat): Y361‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), brain Cellular systems studied:  cell lines, tissue Species studied:  human, mouse

S409-p - TDP-43 (human) ▲

Modsite: GssMDskssGWGM__ SwissProt Entrez-Gene Orthologous residues TDP‑43 (human): S409‑p, TDP‑43 iso4 (human): S293‑p, TDP‑43 (mouse): S409‑p, TDP‑43 (rat): S409‑p Characterization Methods used to characterize site in vivo:  western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  human, mouse Associated Diseases Diseases Alterations Comments ALS increased

S410-p - TDP-43 (human) ▲

Modsite: ssMDskssGWGM___ SwissProt Entrez-Gene Orthologous residues TDP‑43 (human): S410‑p, TDP‑43 iso4 (human): S294‑p, TDP‑43 (mouse): S410‑p, TDP‑43 (rat): S410‑p Characterization Methods used to characterize site in vivo:  western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  human, mouse Associated Diseases Diseases Alterations Comments ALS increased