Curated Information
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Home > Curated Information Page > PubMed Id: 27292631
Spangle JM, et al. (2016) PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer. Cell Rep 15, 2692-704 27292631
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K4-m3 - H3 (human)
Modsite: ____ArtkQtArkst SwissProt Entrez-Gene
Orthologous residues
H3 (human): K4‑m3, H3 (mouse): K4‑m3, H3 iso2 (mouse): K4‑m3, H3 iso3 (mouse): K4‑m3, H3 (rat): K4‑m3, H3 iso3 (rat): K4‑m3, H3 (pig): K4‑m3, H3 (chicken): K4‑m3, H3 (cow): K4‑m3
Characterization
Methods used to characterize site in vivo modification-specific antibody, western blotting
Disease tissue studied:  breast cancer, breast adenocarcinoma, breast ductal carcinoma, breast cancer, triple negative
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HCC38 (breast cell), MDA-MB-361 (breast cell), MDA-MB-468 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MK-2206 decrease
pictilisib decrease
AZD6482 decrease
alpelisib decrease

S225-p - JARID1A (human)
Modsite: TRRVkTQsESGDVSR SwissProt Entrez-Gene
Orthologous residues
JARID1A (human): S225‑p, JARID1A iso2 (human): S225‑p, JARID1A (mouse): S225‑p, JARID1A (rat): S225‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  JARID1A recruitment to the promoters of AKT-regulated genes

T285-p - JARID1A (human)
Modsite: QMRQRKGtLsVNFVD SwissProt Entrez-Gene
Orthologous residues
JARID1A (human): T285‑p, JARID1A iso2 (human): T285‑p, JARID1A (mouse): T285‑p, JARID1A (rat): T285‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  JARID1A recruitment to the promoters of AKT-regulated genes

S287-p - JARID1A (human)
Modsite: RQRKGtLsVNFVDLY SwissProt Entrez-Gene
Orthologous residues
JARID1A (human): S287‑p, JARID1A iso2 (human): S287‑p, JARID1A (mouse): S287‑p, JARID1A (rat): S287‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  JARID1A recruitment to the promoters of AKT-regulated genes

T1225-p - JARID1A (human)
Modsite: SRRPRLEtILSLLVS SwissProt Entrez-Gene
Orthologous residues
JARID1A (human): T1225‑p, JARID1A iso2 (human): T1225‑p, JARID1A (mouse): T1225‑p, JARID1A (rat): T1225‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  JARID1A recruitment to the promoters of AKT-regulated genes

S1255-p - JARID1A (human)
Modsite: CLTERAMsWQDRARQ SwissProt Entrez-Gene
Orthologous residues
JARID1A (human): S1255‑p, JARID1A iso2 (human): S1255‑p, JARID1A (mouse): S1255‑p, JARID1A (rat): S1255‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293T (epithelial), T47D (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  JARID1A recruitment to the promoters of AKT-regulated genes