Park YH, Wood G, Kastner DL, Chae JJ (2016) Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol 17, 914-21 27270401

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S208-p - pyrin (human) ▲

Modsite: VRLRRNAssAGRLQG SwissProt Entrez-Gene Orthologous residues pyrin (human): S208‑p, pyrin (mouse): S205‑p, pyrin (rat): Q204‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  macrophage-bone marrow, mononuclear-blood Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKN2 (human) KINASE PKN1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKN1 (human) phospho-antibody, activation of upstream enzyme, co-immunoprecipitation Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 epsilon (human) Induces co-immunoprecipitation Comments:  turns off pyrin inflammasome activation

S209-p - pyrin (human) ▲

Modsite: RLRRNAssAGRLQGL SwissProt Entrez-Gene Orthologous residues pyrin (human): S209‑p, pyrin (mouse): S206‑p, pyrin (rat): L205‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  macrophage-bone marrow, mononuclear-blood Cellular systems studied:  cell lines Species studied:  human, mouse

S242-p - pyrin (human) ▲

Modsite: SGKMRPRsLEVTIST SwissProt Entrez-Gene Orthologous residues pyrin (human): S242‑p, pyrin (mouse): S241‑p, pyrin (rat): V238‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  macrophage-bone marrow, mononuclear-blood Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKN1 (human) KINASE PKN2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKN1 (human) phospho-antibody, activation of upstream enzyme, co-immunoprecipitation Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 epsilon (human) Induces co-immunoprecipitation Comments:  turns off pyrin inflammasome activation