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Home > Curated Information Page > PubMed Id: 27178468
Chattopadhyay S, et al. (2016) Ubiquitination of the Transcription Factor IRF-3 Activates RIPA, the Apoptotic Pathway that Protects Mice from Viral Pathogenesis. Immunity 44, 1151-61 27178468
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K193-ub - IRF3 (human)
Modsite: GPsENPLkRLLVPGE SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): K193‑ub, IRF3 (mouse): K188‑ub, IRF3 (rat): R189‑ub
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  HT1080 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF31 (human) co-immunoprecipitation, transfection of wild-type enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DHX58 (human) increase
siRNA decrease RNF31 siRNA
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, induced

K313-ub - IRF3 (human)
Modsite: GPDGEVPkDkEGGVF SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): K313‑ub, IRF3 (mouse): K306‑ub, IRF3 (rat): K308‑ub
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  HT1080 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF31 (human) co-immunoprecipitation, transfection of wild-type enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DHX58 (human) increase
siRNA decrease RNF31 siRNA
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, induced

K315-ub - IRF3 (human)
Modsite: DGEVPkDkEGGVFDL SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): K315‑ub, IRF3 (mouse): K308‑ub, IRF3 (rat): K310‑ub
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  HT1080 (fibroblast), MEF (fibroblast)
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE RNF31 (human) co-immunoprecipitation, transfection of wild-type enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DHX58 (human) increase
siRNA decrease RNF31 siRNA
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  apoptosis, induced