Curated Information
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Home > Curated Information Page > PubMed Id: 27175940
Qiu H, et al. (2017) Modification of p27 with O-linked N-acetylglucosamine regulates cell proliferation in hepatocellular carcinoma. Mol Carcinog 56, 258-271 27175940
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S2-gl - p27Kip1 (human)
Modsite: ______MsNVRVSNG SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S2‑gl, p27Kip1 (mouse): S2‑gl, p27Kip1 (rat): S2‑gl
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Effect of modification (process):  cell cycle regulation, cell growth, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Exportin-1 (human) Induces intracellular localization co-immunoprecipitation
Comments:  translocated into the cytoplasm

S10-p - p27Kip1 (human)
Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PUGNAc increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  S10 phosphorylation is regulated by S2 O-GlcNAc

S106-gl - p27Kip1 (human)
Modsite: CKVPAQEsQDVsGSR SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S106‑gl, p27Kip1 (mouse): S106‑gl, p27Kip1 (rat): S106‑gl
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human

S110-gl - p27Kip1 (human)
Modsite: AQEsQDVsGSRPAAP SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S110‑gl, p27Kip1 (mouse): S110‑gl, p27Kip1 (rat): S110‑gl
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human

T157-gl - p27Kip1 (human)
Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T157‑gl, p27Kip1 (mouse): A157‑gl, p27Kip1 (rat): A157‑gl
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human

T198-gl - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑gl, p27Kip1 (mouse): T197‑gl, p27Kip1 (rat): T197‑gl
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human