Li H, et al. (2016) Regulation of Connexin43 Function and Expression by Tyrosine Kinase 2. J Biol Chem 291, 15867-80 27235399

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y247-p - GJA1 (rat) ▲

Modsite: VKGRsDPyHATTGPL SwissProt Entrez-Gene Orthologous residues GJA1 (human): Y247‑p, GJA1 (mouse): Y247‑p, GJA1 (rat): Y247‑p, GJA1 (rabbit): Y247‑p, GJA1 (pig): Y247‑p, GJA1 (hamster): Y247‑p, GJA1 (cow): Y248‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Enzymes shown to modify site in vitro:  Type Enzyme KINASE Tyk2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Tyk2 (human) phospho-antibody, siRNA inhibition of enzyme, transfection of constitutively active enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes angiotensin_2 increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays TUBB (human) Disrupts pull-down assay

Y265-p - GJA1 (rat) ▲

Modsite: kDCGsPkyAyFNGCS SwissProt Entrez-Gene Orthologous residues GJA1 (human): Y265‑p, GJA1 (mouse): Y265‑p, GJA1 (rat): Y265‑p, GJA1 (rabbit): Y265‑p, GJA1 (pig): Y265‑p, GJA1 (hamster): Y265‑p, GJA1 (cow): Y266‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Enzymes shown to modify site in vitro:  Type Enzyme KINASE Tyk2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Tyk2 (human) phospho-antibody, siRNA inhibition of enzyme, transfection of constitutively active enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes angiotensin_2 increase

Y267-p - GJA1 (rat) ▲

Modsite: CGsPkyAyFNGCSSP SwissProt Entrez-Gene Orthologous residues GJA1 (human): Y267‑p, GJA1 (mouse): Y267‑p, GJA1 (rat): Y267‑p, GJA1 (rabbit): Y267‑p, GJA1 (pig): Y267‑p, GJA1 (hamster): Y267‑p, GJA1 (cow): Y268‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Enzymes shown to modify site in vitro:  Type Enzyme KINASE Tyk2 (human)

S279-p - GJA1 (rat) ▲

Modsite: SSPTAPLsPMsPPGY SwissProt Entrez-Gene Orthologous residues GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Tyk2 (human) increase increased by active Tyk2, inhibited by Yyk2 siRNA angiotensin_2 increase

S282-p - GJA1 (rat) ▲

Modsite: TAPLsPMsPPGYkLV SwissProt Entrez-Gene Orthologous residues GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Tyk2 (human) increase increased by active Tyk2, inhibited by Yyk2 siRNA angiotensin_2 increase

Y313-p - GJA1 (rat) ▲

Modsite: sEQNWANysAEQNRM SwissProt Entrez-Gene Orthologous residues GJA1 (human): Y313‑p, GJA1 (mouse): Y313‑p, GJA1 (rat): Y313‑p, GJA1 (rabbit): Y313‑p, GJA1 (pig): Y313‑p, GJA1 (hamster): Y313‑p, GJA1 (cow): Y314‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Enzymes shown to modify site in vitro:  Type Enzyme KINASE Tyk2 (human)

S368-p - GJA1 (rat) ▲

Modsite: QRPssRAssRAssRP SwissProt Entrez-Gene Orthologous residues GJA1 (human): S368‑p, GJA1 (mouse): S368‑p, GJA1 (rat): S368‑p, GJA1 (rabbit): S368‑p, GJA1 (pig): S368‑p, GJA1 (hamster): S368‑p, GJA1 (cow): S369‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, peptide sequencing, western blotting Relevant cell lines - cell types - tissues:  NRK (fibroblast) Cellular systems studied:  cell lines Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes angiotensin_2 increase