Curated Information
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Home > Curated Information Page > PubMed Id: 27228149
Gau D, et al. (2016) Threonine 89 Is an Important Residue of Profilin-1 That Is Phosphorylatable by Protein Kinase A. PLoS One 11, e0156313 27228149
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S57-p - profilin 1 (mouse)
Modsite: VLVGkDRssFFVNGL SwissProt Entrez-Gene
Orthologous residues
profilin 1 (human): S57‑p, profilin 1 (mouse): S57‑p, profilin 1 (rat): S57‑p, profilin 1 (cow): S57‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (cow)

S58-p - profilin 1 (mouse)
Modsite: LVGkDRssFFVNGLt SwissProt Entrez-Gene
Orthologous residues
profilin 1 (human): S58‑p, profilin 1 (mouse): S58‑p, profilin 1 (rat): S58‑p, profilin 1 (cow): S58‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

T90-p - profilin 1 (mouse)
Modsite: EFTMDLRtKstGGAP SwissProt Entrez-Gene
Orthologous residues
profilin 1 (human): T90‑p, profilin 1 (mouse): T90‑p, profilin 1 (rat): T90‑p, profilin 1 (cow): T90‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (cow)
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein conformation
Effect of modification (process):  cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ACTB (mouse) Induces cytoskeletal reorganization microscopy-colocalization
Comments:  reduces overall actin polymerization in cells

S92-p - profilin 1 (mouse)
Modsite: TMDLRtKstGGAPTF SwissProt Entrez-Gene
Orthologous residues
profilin 1 (human): S92‑p, profilin 1 (mouse): S92‑p, profilin 1 (rat): S92‑p, profilin 1 (cow): S92‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (cow)

T93-p - profilin 1 (mouse)
Modsite: MDLRtKstGGAPTFN SwissProt Entrez-Gene
Orthologous residues
profilin 1 (human): T93‑p, profilin 1 (mouse): T93‑p, profilin 1 (rat): T93‑p, profilin 1 (cow): T93‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (cow)