Curated Information
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Home > Curated Information Page > PubMed Id: 15851026
Ma L, et al. (2005) Phosphorylation and functional inactivation of TSC2 by Erk implications for tuberous sclerosis and cancer pathogenesis. Cell 121, 179-93 15851026
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
U0126 phorbol_ester inhibit treatment-induced increase

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
U0126 phorbol_ester inhibit treatment-induced increase

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
U0126 phorbol_ester inhibit treatment-induced increase

S540-p - TSC2 (human)
Modsite: KVMARSLsPPPELEE SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S540‑p, TSC2 iso2 (human): S540‑p, TSC2 iso3 (human): S540‑p, TSC2 iso4 (human): S540‑p, TSC2 (mouse): S540‑p, TSC2 iso6 (mouse): S540‑p, TSC2 (rat): S540‑p, TSC2 iso2 (rat): S540‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S664-p - TSC2 (human)
Modsite: KkTSGPLsPPTGPPG SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S664‑p, TSC2 iso2 (human): S664‑p, TSC2 iso3 (human): S664‑p, TSC2 iso4 (human): S664‑p, TSC2 (mouse): S664‑p, TSC2 iso6 (mouse): S664‑p, TSC2 (rat): S664‑p, TSC2 iso2 (rat): S664‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TSC1 (human) Disrupts electrophoretic visualization, co-immunoprecipitation