Curated Information
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Home > Curated Information Page > PubMed Id: 12766774
Bulavin DV, et al. (2003) Dual phosphorylation controls Cdc25 phosphatases and mitotic entry. Nat Cell Biol 5, 545-51 12766774
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S307-p - CDC25B iso2 (human)
Modsite: KCQRLFRsPsMPCSV SwissProt Entrez-Gene
Orthologous residues
CDC25B (human): S321‑p, CDC25B iso2 (human): S307‑p, CDC25B iso3 (human): S280‑p, CDC25B (mouse): S319‑p, CDC25B (rat): S317‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)

S309-p - CDC25B iso2 (human)
Modsite: QRLFRsPsMPCSVIR SwissProt Entrez-Gene
Orthologous residues
CDC25B (human): S323‑p, CDC25B iso2 (human): S309‑p, CDC25B iso3 (human): S282‑p, CDC25B (mouse): S321‑p, CDC25B (rat): S319‑p

S214-p - CDC25C (human)
Modsite: sRsGLyRsPsMPENL SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S214‑p, CDC25C iso3 (human): S171‑p, CDC25C (mouse): , CDC25C (frog): S285‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Disrupts not reported
Comments:  In the absence of S214 phosphorylation Cdc25C can be phosphorylated on S216 and can bind to 14-3-3 proteins in mitosis

S216-p - CDC25C (human)
Modsite: sGLyRsPsMPENLNR SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S216‑p, CDC25C iso3 (human): S173‑p, CDC25C (mouse): , CDC25C (frog): S287‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Chk1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation no change compared to control
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation

Y15-p - CDK1 (human)
Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation no change compared to control