Kahle KT, et al. (2016) Inhibition of the kinase WNK1/HSN2 ameliorates neuropathic pain by restoring GABA inhibition. Sci Signal 9, ra32 27025876

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T906-p - KCC2 iso2 (mouse) ▲

Modsite: SAYTYEKtLVMEQRS SwissProt Entrez-Gene Orthologous residues KCC2 (human): T929‑p, KCC2 iso2 (human): T906‑p, KCC2 (mouse): T929‑p, KCC2 iso2 (mouse): T906‑p, KCC2 (rat): T929‑p, KCC2 iso2 (rat): T906‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SNI increase Downstream Regulation Effect of modification (function):  activity, inhibited

T1008-p - KCC2 iso2 (mouse) ▲

Modsite: PEVHLtWtKDKSVAE SwissProt Entrez-Gene Orthologous residues KCC2 (human): T1032‑p, KCC2 iso2 (human): T1009‑p, KCC2 (mouse): T1031‑p, KCC2 iso2 (mouse): T1008‑p, KCC2 (rat): T1032‑p, KCC2 iso2 (rat): T1009‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SNI increase Downstream Regulation Effect of modification (function):  activity, inhibited

T196-p - NKCC1 (mouse) ▲

Modsite: HQQYYYDtHTNtYYL SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T203‑p, NKCC1 iso3 (human): T203‑p, NKCC1 (mouse): T196‑p, NKCC1 (rat): T194‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SNI no change compared to control WNK1/HSN2 knockout

T200-p - NKCC1 (mouse) ▲

Modsite: YYDtHTNtYYLRtFG SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T207‑p, NKCC1 iso3 (human): T207‑p, NKCC1 (mouse): T200‑p, NKCC1 (rat): T198‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SNI no change compared to control WNK1/HSN2 knockout

T205-p - NKCC1 (mouse) ▲

Modsite: TNtYYLRtFGHNtMD SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T212‑p, NKCC1 iso3 (human): T212‑p, NKCC1 (mouse): T205‑p, NKCC1 (rat): T203‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SNI no change compared to control WNK1/HSN2 knockout

S383-p - STLK3 (mouse) ▲

Modsite: VRRVPGssGHLHKTE SwissProt Entrez-Gene Orthologous residues STLK3 (human): S371‑p, STLK3 (mouse): S383‑p, STLK3 (rat): S380‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  spinal cord Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SNI no change compared to control WNK1/HSN2 knockout