Curated Information
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Home > Curated Information Page > PubMed Id: 26942675
Li X, et al. (2016) Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. Mol Cell 61, 705-19 26942675
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S293-p - PDHA1 (human)
Modsite: TYRyHGHsMsDPGVs SwissProt Entrez-Gene
Orthologous residues
PDHA1 (human): S293‑p, PDHA1 iso2 (human): S300‑p, PDHA1 iso4 (human): S331‑p, PDHA1 (mouse): S293‑p, PDHA1 (rat): S293‑p, PDHA1 (pig): S292‑p

Y243-p - PDHK1 (human)
Modsite: ARRLCDLyyINSPEL SwissProt Entrez-Gene
Orthologous residues
PDHK1 (human): Y243‑p, PDHK1 (mouse): Y241‑p, PDHK1 (rat): Y241‑p

T338-p - PDHK1 (human)
Modsite: LFNYMyStAPRPRVE SwissProt Entrez-Gene
Orthologous residues
PDHK1 (human): T338‑p, PDHK1 (mouse): T336‑p, PDHK1 (rat): T336‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PGK1 (human) phosphoaminoacid analysis, transfection of inactive enzyme, transfection of wild-type enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  carcinogenesis, induced, cell growth, induced
Comments:  promotes lactate production, brain tumorigenesis

S203-p - PGK1 (human)
Modsite: yFAkALEsPERPFLA SwissProt Entrez-Gene
Orthologous residues
PGK1 (human): S203‑p, PGK1 (mouse): S203‑p, PGK1 (rat): S203‑p, PGK1 (cow): S203‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, phosphoamino acid analysis, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia increase
U0126 hypoxia inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization, protein conformation
Comments:  mitochondrial translocation of PGK1, PIN1 speci¿cally isomerizes the PGK1 pS203/P204 motif

T378-p - PGK1 (human)
Modsite: IGGGDTAtCCAkWNT SwissProt Entrez-Gene
Orthologous residues
PGK1 (human): T378‑p, PGK1 (mouse): T378‑p, PGK1 (rat): T378‑p, PGK1 (cow): T378‑p