Lee SB, et al. (2016) An ID2-dependent mechanism for VHL inactivation in cancer. Nature 529, 172-7 26735018

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T27-p - ID2 (human) ▲

Modsite: LGISRSKtPVDDPMS SwissProt Entrez-Gene Orthologous residues ID2 (human): T27‑p, ID2 (mouse): T27‑p, ID2 (rat): T27‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  'stem, neural', U87MG (glial) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE DYRK1B (human) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypoxia decrease EGLN2 (human) increase

T27-p - ID2 (mouse) ▲

Modsite: LGISRsKtPVDDPMS SwissProt Entrez-Gene Orthologous residues ID2 (human): T27‑p, ID2 (mouse): T27‑p, ID2 (rat): T27‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  'stem, neural', U87MG (glial) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE DYRK1A (human) phospho-antibody, co-immunoprecipitation, transfection of wild-type enzyme, siRNA inhibition of enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell growth, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays VHL (mouse) Disrupts pull-down assay