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Home > Curated Information Page > PubMed Id: 26887952
Sassa T, Hirayama T, Kihara A (2016) Enzyme Activities of the Ceramide Synthases CERS2-6 Are Regulated by Phosphorylation in the C-terminal Region. J Biol Chem 291, 7477-87 26887952
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S341-p - LASS2 (human)
Modsite: kLVEDERsDREEtEs SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): S341‑p, LASS2 (mouse): S341‑p, LASS2 (rat): S341‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T346-p - LASS2 (human)
Modsite: ERsDREEtEssEGEE SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): T346‑p, LASS2 (mouse): T346‑p, LASS2 (rat): T346‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S348-p - LASS2 (human)
Modsite: sDREEtEssEGEEAA SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): S348‑p, LASS2 (mouse): S348‑p, LASS2 (rat): S348‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S349-p - LASS2 (human)
Modsite: DREEtEssEGEEAAA SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): S349‑p, LASS2 (mouse): S349‑p, LASS2 (rat): S349‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S340-p - LASS3 (human)
Modsite: KSIQDVRsDDEDYEE SwissProt Entrez-Gene
Orthologous residues
LASS3 (human): S340‑p, LASS3 (mouse): S340‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S342-p - LASS4 (human)
Modsite: QMEKDIRsDVEEsDs SwissProt Entrez-Gene
Orthologous residues
LASS4 (human): S342‑p, LASS4 (mouse): S342‑p, LASS4 (rat): S342‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S347-p - LASS4 (human)
Modsite: IRsDVEEsDssEEAA SwissProt Entrez-Gene
Orthologous residues
LASS4 (human): S347‑p, LASS4 (mouse): P347‑p, LASS4 (rat): S347‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S349-p - LASS4 (human)
Modsite: sDVEEsDssEEAAAA SwissProt Entrez-Gene
Orthologous residues
LASS4 (human): S349‑p, LASS4 (mouse): S349‑p, LASS4 (rat): S349‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S350-p - LASS4 (human)
Modsite: DVEEsDssEEAAAAQ SwissProt Entrez-Gene
Orthologous residues
LASS4 (human): S350‑p, LASS4 (mouse): S350‑p, LASS4 (rat): S350‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S350-p - LASS5 (human)
Modsite: KVSKDDRsDVEsssE SwissProt Entrez-Gene
Orthologous residues
LASS5 (human): S350‑p, LASS5 (mouse): S350‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S354-p - LASS5 (human)
Modsite: DDRsDVEsssEEEDV SwissProt Entrez-Gene
Orthologous residues
LASS5 (human): S354‑p, LASS5 (mouse): S354‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S355-p - LASS5 (human)
Modsite: DRsDVEsssEEEDVT SwissProt Entrez-Gene
Orthologous residues
LASS5 (human): S355‑p, LASS5 (mouse): S355‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S356-p - LASS5 (human)
Modsite: RsDVEsssEEEDVTT SwissProt Entrez-Gene
Orthologous residues
LASS5 (human): S356‑p, LASS5 (mouse): S356‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S341-p - LASS6 (human)
Modsite: KVSKDDRsDIEsssD SwissProt Entrez-Gene
Orthologous residues
LASS6 (human): S341‑p, LASS6 (mouse): S341‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S345-p - LASS6 (human)
Modsite: DDRsDIEsssDEEDS SwissProt Entrez-Gene
Orthologous residues
LASS6 (human): S345‑p, LASS6 (mouse): S345‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S346-p - LASS6 (human)
Modsite: DRsDIEsssDEEDSE SwissProt Entrez-Gene
Orthologous residues
LASS6 (human): S346‑p, LASS6 (mouse): S346‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S347-p - LASS6 (human)
Modsite: RsDIEsssDEEDSEP SwissProt Entrez-Gene
Orthologous residues
LASS6 (human): S347‑p, LASS6 (mouse): S347‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, electrophoretic mobility shift
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S341-p - LASS2 (mouse)
Modsite: KLIEDERsDREEtEs SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): S341‑p, LASS2 (mouse): S341‑p, LASS2 (rat): S341‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T346-p - LASS2 (mouse)
Modsite: ERsDREEtEssEGEE SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): T346‑p, LASS2 (mouse): T346‑p, LASS2 (rat): T346‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S348-p - LASS2 (mouse)
Modsite: sDREEtEssEGEEtA SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): S348‑p, LASS2 (mouse): S348‑p, LASS2 (rat): S348‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S349-p - LASS2 (mouse)
Modsite: DREEtEssEGEEtAA SwissProt Entrez-Gene
Orthologous residues
LASS2 (human): S349‑p, LASS2 (mouse): S349‑p, LASS2 (rat): S349‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced