Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 26887051
Booth L, et al. (2016) Multi-kinase inhibitors can associate with heat shock proteins through their NH2-termini by which they suppress chaperone function. Oncotarget 7, 12975-96 26887051
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 no change compared to control

S13-p - CDC37 (human)
Modsite: VWDHIEVsDDEDETH SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): S13‑p, CDC37 (mouse): S13‑p, CDC37 (rat): S13‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 decrease peri-nuclear staining of both ULK-1 and ULK-1 S757
OSU-03012, sildenafil decrease

S15-p - HSP27 (human)
Modsite: FsLLrGPsWDPFrDW SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S15‑p, HSP27 (mouse): S15‑p, HSP27 (rat): S15‑p, HSP27 (pig): S15‑p, HSP27 (hamster): S15‑p, HSP27 (chicken): S15‑p, HSP27 (dog): S15‑p, HSP27 (cow): S15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 increase

S78-p - HSP27 (human)
Modsite: PAysRALsRQLssGV SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S78‑p, HSP27 (mouse): N82‑p, HSP27 (rat): N82‑p, HSP27 (pig): S80‑p, HSP27 (hamster): N86‑p, HSP27 (chicken): S77‑p, HSP27 (dog): S82‑p, HSP27 (cow): S74‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 no change compared to control

S82-p - HSP27 (human)
Modsite: RALsRQLssGVsEIr SwissProt Entrez-Gene
Orthologous residues
HSP27 (human): S82‑p, HSP27 (mouse): S86‑p, HSP27 (rat): S86‑p, HSP27 (pig): S84‑p, HSP27 (hamster): S90‑p, HSP27 (chicken): S80‑p, HSP27 (dog): S86‑p, HSP27 (cow): S78‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 no change compared to control

S2448-p - mTOR (human)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 decrease peri-nuclear staining of both ULK-1 and ULK-1 S757

S758-p - ULK1 (human)
Modsite: PVVFtVGsPPsGStP SwissProt Entrez-Gene
Orthologous residues
ULK1 (human): S758‑p, ULK1 (mouse): S757‑p, ULK1 (rat): S757‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  glial-brain
Cellular systems studied:  cell lines
Species studied:  human
Comments:  primary glioblastoma
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
OSU-03012 decrease peri-nuclear staining of both ULK-1 and ULK-1 S757