Booth L, et al. (2016) Multi-kinase inhibitors can associate with heat shock proteins through their NH2-termini by which they suppress chaperone function. Oncotarget 7, 12975-96 26887051

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T308-p - Akt1 (human) ▲

Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene Orthologous residues Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 no change compared to control

S13-p - CDC37 (human) ▲

Modsite: VWDHIEVsDDEDETH SwissProt Entrez-Gene Orthologous residues CDC37 (human): S13‑p, CDC37 (mouse): S13‑p, CDC37 (rat): S13‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 decrease peri-nuclear staining of both ULK-1 and ULK-1 S757 OSU-03012, sildenafil decrease

S15-p - HSP27 (human) ▲

Modsite: FsLLrGPsWDPFrDW SwissProt Entrez-Gene Orthologous residues HSP27 (human): S15‑p, HSP27 (mouse): S15‑p, HSP27 (rat): S15‑p, HSP27 (pig): S15‑p, HSP27 (hamster): S15‑p, HSP27 (chicken): S15‑p, HSP27 (dog): S15‑p, HSP27 (cow): S15‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 increase

S78-p - HSP27 (human) ▲

Modsite: PAysRALsRQLssGV SwissProt Entrez-Gene Orthologous residues HSP27 (human): S78‑p, HSP27 (mouse): N82‑p, HSP27 (rat): N82‑p, HSP27 (pig): S80‑p, HSP27 (hamster): N86‑p, HSP27 (chicken): S77‑p, HSP27 (dog): S82‑p, HSP27 (cow): S74‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 no change compared to control

S82-p - HSP27 (human) ▲

Modsite: RALsRQLssGVsEIr SwissProt Entrez-Gene Orthologous residues HSP27 (human): S82‑p, HSP27 (mouse): S86‑p, HSP27 (rat): S86‑p, HSP27 (pig): S84‑p, HSP27 (hamster): S90‑p, HSP27 (chicken): S80‑p, HSP27 (dog): S86‑p, HSP27 (cow): S78‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 no change compared to control

S2448-p - mTOR (human) ▲

Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene Orthologous residues mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 decrease peri-nuclear staining of both ULK-1 and ULK-1 S757

S758-p - ULK1 (human) ▲

Modsite: PVVFtVGsPPsGStP SwissProt Entrez-Gene Orthologous residues ULK1 (human): S758‑p, ULK1 (mouse): S757‑p, ULK1 (rat): S757‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  brain cancer, glioblastoma, glioma Relevant cell lines - cell types - tissues:  glial-brain Cellular systems studied:  cell lines Species studied:  human Comments:  primary glioblastoma Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes OSU-03012 decrease peri-nuclear staining of both ULK-1 and ULK-1 S757