He CL, et al. (2016) Pyruvate Kinase M2 Activates mTORC1 by Phosphorylating AKT1S1. Sci Rep 6, 21524 26876154

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

T37-p - 4E-BP1 (human) ▲

Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKM (human) increase rapamycin PKM (human) inhibit treatment-induced increase

T46-p - 4E-BP1 (human) ▲

Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKM (human) increase rapamycin PKM (human) inhibit treatment-induced increase

T412-p - p70S6K (human) ▲

Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PKM (human) increase rapamycin PKM (human) inhibit treatment-induced increase

S202-p - PRAS40 (human) ▲

Modsite: EKRtEARssDEENGP SwissProt Entrez-Gene Orthologous residues PRAS40 (human): S202‑p, PRAS40 iso2 (human): S72‑p, PRAS40 iso3 (human): S222‑p, PRAS40 (mouse): S203‑p, PRAS40 (rat): S203‑p, PRAS40 (cow): S202‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKM (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKM (human) transfection of wild-type enzyme, co-immunoprecipitation, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  autophagy, inhibited, cell growth, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 beta (human) Induces co-immunoprecipitation Raptor (human) Disrupts co-immunoprecipitation Comments:  activates mTORC1

S203-p - PRAS40 (human) ▲

Modsite: KRtEARssDEENGPP SwissProt Entrez-Gene Orthologous residues PRAS40 (human): S203‑p, PRAS40 iso2 (human): S73‑p, PRAS40 iso3 (human): S223‑p, PRAS40 (mouse): S204‑p, PRAS40 (rat): S204‑p, PRAS40 (cow): S203‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKM (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKM (human) transfection of wild-type enzyme, co-immunoprecipitation, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  autophagy, inhibited, cell growth, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Raptor (human) Disrupts co-immunoprecipitation 14-3-3 beta (human) Induces co-immunoprecipitation Comments:  activates mTORC1