Curated Information
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Home > Curated Information Page > PubMed Id: 26862730
Lim HC, Jou TS (2016) Ras-activated RSK1 phosphorylates EBP50 to regulate its nuclear localization and promote cell proliferation. Oncotarget 7, 10283-96 26862730
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T156-p - NHERF (human)
Modsite: ELRPRLCtMKKGPsG SwissProt Entrez-Gene
Orthologous residues
NHERF (human): T156‑p, NHERF (mouse): T151‑p, NHERF (rat): T153‑p, NHERF (rabbit): A156‑p, NHERF (dog): A163‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE p90RSK (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p90RSK (human) phospho-motif antibody, siRNA inhibition of enzyme, modification site within consensus motif, activation of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell growth, induced
Comments:  increased nuclear localization

S339-p - NHERF (human)
Modsite: ERAHQKRssKRAPQM SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S339‑p, NHERF (mouse): S336‑p, NHERF (rat): S337‑p, NHERF (rabbit): S339‑p, NHERF (dog): S348‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S340-p - NHERF (human)
Modsite: RAHQKRssKRAPQMD SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S340‑p, NHERF (mouse): S337‑p, NHERF (rat): S338‑p, NHERF (rabbit): S340‑p, NHERF (dog): S349‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S380-p - p90RSK (human)
Modsite: HQLFRGFsFVAtGLM SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S380‑p, p90RSK iso2 (human): S389‑p, p90RSK (mouse): S369‑p, p90RSK iso3 (mouse): , p90RSK (rat): S380‑p, p90RSK (chicken): S398‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human