Curated Information
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Home > Curated Information Page > PubMed Id: 26831064
Ertych N, et al. (2016) CHK2-BRCA1 tumor-suppressor axis restrains oncogenic Aurora-A kinase to ensure proper mitotic microtubule assembly. Proc Natl Acad Sci U S A 113, 1817-22 26831064
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T288-p - AurA (human)
Modsite: APSsRRttLCGtLDy SwissProt Entrez-Gene
Orthologous residues
AurA (human): T288‑p, AurA (mouse): T279‑p, AurA (rat): T281‑p, AurA (pig): T288‑p, AurA (frog): T295‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human

S308-p - BRCA1 (human)
Modsite: KAEFCNKskQPGLAR SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S308‑p, BRCA1 iso6 (human): , BRCA1 iso7 (human): S308‑p, BRCA1 (mouse): S305‑p, BRCA1 (rat): S306‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AurA (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme

S988-p - BRCA1 (human)
Modsite: PPLFPIksFVKTKCK SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S988‑p, BRCA1 iso6 (human): , BRCA1 iso7 (human): S988‑p, BRCA1 (mouse): S971‑p, BRCA1 (rat): S975‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP6C (human) phospho-antibody, co-immunoprecipitation