Olabisi OA, et al. (2016) APOL1 kidney disease risk variants cause cytotoxicity by depleting cellular potassium and inducing stress-activated protein kinases. Proc Natl Acad Sci U S A 113, 830-7 26699492

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T222-p - MAPKAPK2 (human) ▲

Modsite: tSHNsLttPCytPYY SwissProt Entrez-Gene Orthologous residues MAPKAPK2 (human): T222‑p, MAPKAPK2 iso2 (human): T222‑p, MAPKAPK2 (mouse): T208‑p, MAPKAPK2 (rat): T208‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) increase APOL1 risk variants G1 G2

S218-p - MKK3 (human) ▲

Modsite: IsGyLVDsVAKtMDA SwissProt Entrez-Gene Orthologous residues MKK3 (human): S218‑p, MKK3 iso2 (human): S189‑p, MKK3 iso3 (human): S223‑p, MKK3 (mouse): S218‑p, MKK3 (rat): S290‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) increase APOL1 risk variants G1 G2

S207-ac - MKK6 (human) ▲

Modsite: IsGyLVDsVAKtIDA SwissProt Entrez-Gene Orthologous residues MKK6 (human): S207‑ac, MKK6 iso2 (human): S151‑ac, MKK6 (mouse): S207‑ac, MKK6 (rat): S207‑ac Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) increase APOL1 risk variants G1 G2

T211-ac - MKK6 (human) ▲

Modsite: LVDsVAKtIDAGCKP SwissProt Entrez-Gene Orthologous residues MKK6 (human): T211‑ac, MKK6 iso2 (human): T155‑ac, MKK6 (mouse): T211‑ac, MKK6 (rat): T211‑ac Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) increase APOL1 risk variants G1 G2

T180-p - P38A (human) ▲

Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene Orthologous residues P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) increase APOL1 risk variants G1 G2

Y182-p - P38A (human) ▲

Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene Orthologous residues P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) increase APOL1 risk variants G1 G2

Y705-p - STAT3 (human) ▲

Modsite: DPGsAAPyLktKFIC SwissProt Entrez-Gene Orthologous residues STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  Flp-In T-Rex-293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes APOL1 (human) decrease APOL1 risk variants G, G2