Mai H, et al. (2003) A functional role for nicotine in Bcl2 phosphorylation and suppression of apoptosis. J Biol Chem 278, 1886-91 12421819

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T69-p - Bcl-2 (human) ▲

Modsite: SRDPVARtsPLQtPA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): T69‑p, Bcl‑2 (mouse): T69‑p, Bcl‑2 (rat): T69‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Disease tissue studied:  lung cancer, small-cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H82 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nicotine no change compared to control

S70-p - Bcl-2 (human) ▲

Modsite: RDPVARtsPLQtPAA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S70‑p, Bcl‑2 (mouse): S70‑p, Bcl‑2 (rat): S70‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Disease tissue studied:  lung cancer, small-cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H82 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nicotine increase staurosporine nicotine inhibit treatment-induced increase PD98059 nicotine inhibit treatment-induced increase ET18OCH3 nicotine inhibit treatment-induced increase SB202190 nicotine no effect upon treatment-induced increase hexamethionium nicotine no effect upon treatment-induced increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  apoptosis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays BAX (human) Induces co-immunoprecipitation

S87-p - Bcl-2 (human) ▲

Modsite: AAAGPALsPVPPVVH SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S87‑p, Bcl‑2 (mouse): S84‑p, Bcl‑2 (rat): S84‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Disease tissue studied:  lung cancer, small-cell lung cancer Relevant cell lines - cell types - tissues:  NCI-H82 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes nicotine no change compared to control