Curated Information
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Home > Curated Information Page > PubMed Id: 12734207
Sakoda H, et al. (2003) Differing roles of Akt and serum- and glucocorticoid-regulated kinase in glucose metabolism, DNA synthesis, and oncogenic activity. J Biol Chem 278, 25802-7 12734207
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  32D (myeloid), 3T3 (fibroblast), 3T3-L1 (fibroblast), hepatocyte
Cellular systems studied:  cell lines
Species studied:  human

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  32D (myeloid), 3T3 (fibroblast), 3T3-L1 (fibroblast), hepatocyte
Cellular systems studied:  cell lines
Species studied:  human

S21-p - GSK3A (rat)
Modsite: SGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  32D (myeloid), 3T3 (fibroblast), 3T3-L1 (fibroblast), hepatocyte
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
SGK1 (rat) increase K127/T256/422D SGK mutation
Akt1 (rat) no change compared to control myr-Akt
SGK1 (rat) no change compared to control myr-SGK
no change compared to control WT Akt
no change compared to control K179M/T308M/S473M Akt mutant
increase myr-Akt
no change compared to control WT SGK
no change compared to control K127A/256A/S422A SGK mutant
increase S422D SGK mutant
increase myr-SGK mutant

T256-p - SGK1 (rat)
Modsite: EHNGTTstFCGTPEY SwissProt Entrez-Gene
Orthologous residues
SGK1 (human): T256‑p, SGK1 iso2 (human): T351‑p, SGK1 iso3 (human): T270‑p, SGK1 (mouse): T256‑p, SGK1 (rat): T256‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  32D (myeloid), 3T3 (fibroblast), 3T3-L1 (fibroblast), hepatocyte
Cellular systems studied:  cell lines
Species studied:  human

S421-p - SGK1 (rat)
Modsite: AEAFLGFsYAPPMDS SwissProt Entrez-Gene
Orthologous residues
SGK1 (human): S422‑p, SGK1 iso2 (human): S517‑p, SGK1 iso3 (human): S436‑p, SGK1 (mouse): S422‑p, SGK1 (rat): S421‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  32D (myeloid), 3T3 (fibroblast), 3T3-L1 (fibroblast), hepatocyte
Cellular systems studied:  cell lines
Species studied:  human