Lornejad-Schäfer MR, et al. (2003) Osmotic regulation of insulin-induced mitogen-activated protein kinase phosphatase (MKP-1) expression in H4IIE rat hepatoma cells. Biochem J 371, 609-19 12529177

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S473-p - Akt1 (rat) ▲

Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  H4IIe (hepatic) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase wortmannin insulin inhibit treatment-induced increase LY294002 insulin inhibit treatment-induced increase osmotic_stress insulin no effect upon treatment-induced increase calyculin_A, osmotic_stress insulin no effect upon treatment-induced increase vanadate, osmotic_stress insulin no effect upon treatment-induced increase

T412-p - p70S6K (rat) ▲

Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  H4IIe (hepatic) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase wortmannin insulin inhibit treatment-induced increase osmotic_stress insulin no effect upon treatment-induced increase calyculin_A, osmotic_stress insulin inhibit treatment-induced increase vanadate, osmotic_stress insulin inhibit treatment-induced increase

T444-p - p70S6K (rat) ▲

Modsite: RFIGsPRtPVsPVKF SwissProt Entrez-Gene Orthologous residues p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly): ‑ Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  H4IIe (hepatic) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase wortmannin insulin inhibit treatment-induced increase osmotic_stress insulin no effect upon treatment-induced increase calyculin_A, osmotic_stress insulin inhibit treatment-induced increase vanadate, osmotic_stress insulin inhibit treatment-induced increase

S447-p - p70S6K (rat) ▲

Modsite: GsPRtPVsPVKFsPG SwissProt Entrez-Gene Orthologous residues p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): ‑ Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  H4IIe (hepatic) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase wortmannin insulin inhibit treatment-induced increase osmotic_stress insulin no effect upon treatment-induced increase calyculin_A, osmotic_stress insulin inhibit treatment-induced increase vanadate, osmotic_stress insulin inhibit treatment-induced increase

T410-p - PKCZ (rat) ▲

Modsite: GPGDTTStFCGTPNY SwissProt Entrez-Gene Orthologous residues PKCZ (human): T410‑p, PKCZ iso2 (human): T227‑p, PKCZ iso3 (human): T306‑p, PKCZ (mouse): T410‑p, PKCZ (rat): T410‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  H4IIe (hepatic) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase osmotic_stress insulin no effect upon treatment-induced increase PKCI_19-36 insulin inhibit treatment-induced increase