Curated Information
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Home > Curated Information Page > PubMed Id: 15159397
Fabbro M, et al. (2004) BRCA1-BARD1 complexes are required for p53Ser-15 phosphorylation and a G1/S arrest following ionizing radiation-induced DNA damage. J Biol Chem 279, 31251-8 15159397
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1981-p - ATM (human)
Modsite: sLAFEEGsQSTtIss SwissProt Entrez-Gene
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p, ATM (rat): S1988‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV no change compared to control

S1423-p - BRCA1 (human)
Modsite: AVLEQHGsQPSNSYP SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1423‑p, BRCA1 iso6 (human): S320‑p, BRCA1 iso7 (human): S1423‑p, BRCA1 (mouse): N1379‑p, BRCA1 (rat): S1380‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  phosphorylation of BRCA1 at S1423 and S1524 is required for ATM/ATR-mediated p53 S15 phosphorylation.

S1524-p - BRCA1 (human)
Modsite: LQNRNYPsQEELIKV SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1524‑p, BRCA1 iso6 (human): S420‑p, BRCA1 iso7 (human): S1545‑p, BRCA1 (mouse): S1481‑p, BRCA1 (rat): S1482‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  phosphorylation of BRCA1 at S1423 and S1524 is required for ATM/ATR-mediated p53 S15 phosphorylation.

S317-p - Chk1 (human)
Modsite: ENVkYsssQPEPRTG SwissProt Entrez-Gene
Orthologous residues
Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing_radiation increase

T68-p - Chk2 (human)
Modsite: SsLEtVstQELYsIP SwissProt Entrez-Gene
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso9 (human): T68‑p, Chk2 iso11 (human): , Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV no change compared to control

S139-p - H2AX (human)
Modsite: GkkAtQAsQEy____ SwissProt Entrez-Gene
Orthologous residues
H2AX (human): S139‑p, H2AX (mouse): S139‑p, H2AX (rat): S139‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase

S63-p - Jun (human)
Modsite: kNsDLLtsPDVGLLk SwissProt Entrez-Gene
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p, Jun (cow): S63‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation no change compared to control
UV increase

S15-p - p53 (human)
Modsite: PsVEPPLsQEtFsDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HCC1937 (breast cell), HEK293T (epithelial), MCF-7 (breast cell), U2OS (bone cell) [GR (human)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation BRCA1 (human), BARD1 (human) increase
UV BRCA1 (human), BARD1 (human) increase