Curated Information
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Home > Curated Information Page > PubMed Id: 26590425
Wang MT, et al. (2015) K-Ras Promotes Tumorigenicity through Suppression of Non-canonical Wnt Signaling. Cell 163, 1237-51 26590425
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T286-p - CAMK2A (mouse)
Modsite: sCMHRQEtVDCLkkF SwissProt Entrez-Gene
Orthologous residues
CAMK2A (human): T286‑p, CAMK2A iso2 (human): T286‑p, CAMK2A (mouse): T286‑p, CAMK2A iso2 (mouse): , CAMK2A (rat): T286‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (mouse) increase
FZD8 (mouse) HRas (mouse) increase shFZD8 inhibits
KRas (mouse) decrease
prostratin increase

S33-p - CTNNB1 (mouse)
Modsite: QQQsYLDsGIHsGAT SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (mouse) increase
KRas (mouse) decrease

S37-p - CTNNB1 (mouse)
Modsite: YLDsGIHsGATtTAP SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (mouse) increase
KRas (mouse) decrease

T41-p - CTNNB1 (mouse)
Modsite: GIHsGATtTAPsLSG SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): T41‑p, CTNNB1 (mouse): T41‑p, CTNNB1 (rat): T41‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (mouse) increase
KRas (mouse) decrease

S181-p - KRas iso2 (mouse)
Modsite: GKKKKKKsRTRCTVM SwissProt Entrez-Gene
Orthologous residues
KRas (human): , KRas iso2 (human): S181‑p, KRas (mouse): , KRas iso2 (mouse): S181‑p, KRas (rat):
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Calmodulin (mouse) Disrupts co-immunoprecipitation
Comments:  increase in Fzd8 promoter activity, and of NF-AT transcription