Chen C, et al. (2016) Determination of sites of U50,488H-promoted phosphorylation of the mouse κ opioid receptor (KOPR): disconnect between KOPR phosphorylation and internalization. Biochem J 473, 497-508 26635353

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S356-p - KOR-1 (mouse) ▲

Modsite: KMRMERQstNRVRNt SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): S356‑p, KOR‑1 (mouse): S356‑p, KOR‑1 (rat): S356‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase norBNI U50488 inhibit treatment-induced increase etorphine increase Downstream Regulation Effect of modification (function):  intracellular localization

T357-p - KOR-1 (mouse) ▲

Modsite: MRMERQstNRVRNtV SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): T357‑p, KOR‑1 (mouse): T357‑p, KOR‑1 (rat): T357‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase norBNI U50488 inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  intracellular localization

T363-p - KOR-1 (mouse) ▲

Modsite: stNRVRNtVQDPAsM SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): T363‑p, KOR‑1 (mouse): T363‑p, KOR‑1 (rat): T363‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase norBNI U50488 inhibit treatment-induced increase etorphine increase Downstream Regulation Effect of modification (function):  intracellular localization, phosphorylation Comments:  important for phosphorylation of S369

S369-p - KOR-1 (mouse) ▲

Modsite: NtVQDPAsMRDVGGM SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): Y369‑p, KOR‑1 (mouse): S369‑p, KOR‑1 (rat): S369‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site, phospho-antibody Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  Neuro-2a (neuron) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase norBNI U50488 inhibit treatment-induced increase etorphine increase Downstream Regulation Effect of modification (function):  intracellular localization