Curated Information
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Home > Curated Information Page > PubMed Id: 25014166
Czaplinska D, et al. (2014) Phosphorylation of RSK2 at Tyr529 by FGFR2-p38 enhances human mammary epithelial cells migration. Biochim Biophys Acta 1843, 2461-70 25014166
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T359-p - p90RSK (human)
Modsite: DTEFTSRtPKDsPGI SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T359‑p, p90RSK iso2 (human): T368‑p, p90RSK (mouse): T348‑p, p90RSK iso3 (mouse): , p90RSK (rat): T359‑p, p90RSK (chicken): T377‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer, breast adenocarcinoma
Relevant cell lines - cell types - tissues:  MCF10A1 (epithelial), MDA-MB-361 (breast cell), SKBr3 (breast cell)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FGF2 increase

S363-p - p90RSK (human)
Modsite: TSRtPKDsPGIPPsA SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S363‑p, p90RSK iso2 (human): S372‑p, p90RSK (mouse): S352‑p, p90RSK iso3 (mouse): , p90RSK (rat): S363‑p, p90RSK (chicken): S381‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer, breast adenocarcinoma
Relevant cell lines - cell types - tissues:  MCF10A1 (epithelial), MDA-MB-361 (breast cell), SKBr3 (breast cell)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FGF2 increase

T573-p - p90RSK (human)
Modsite: AENGLLMtPCytANF SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T573‑p, p90RSK iso2 (human): T582‑p, p90RSK (mouse): T562‑p, p90RSK iso3 (mouse): , p90RSK (rat): T573‑p, p90RSK (chicken): T590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer, breast adenocarcinoma
Relevant cell lines - cell types - tissues:  MCF10A1 (epithelial), MDA-MB-361 (breast cell), SKBr3 (breast cell)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FGF2 increase

Y529-p - RSK2 (human)
Modsite: TITKTVEyLHAQGVV SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y529‑p, RSK2 (mouse): Y529‑p, RSK2 (rat): Y529‑p, RSK2 (cow): Y529‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  breast cancer, breast adenocarcinoma
Relevant cell lines - cell types - tissues:  MCF10A1 (epithelial), MDA-MB-361 (breast cell), SKBr3 (breast cell)
Cellular systems studied:  cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SB202190 decrease
U0126 no change compared to control
SU6656 no change compared to control
FGF2 increase