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Home > Curated Information Page > PubMed Id: 10867021
Katagiri T, et al. (2000) Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J Biol Chem 275, 19645-52 10867021
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site as compared to WT
anti-CD3/CD28 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site compared to WT

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site as compared to WT
anti-CD3/CD28 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site compared to WT

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site as compared to WT
anti-CD3/CD28 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site compared to WT

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site as compared to WT
anti-CD3/CD28 no change compared to control no change in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site compared to WT

T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
anti-CD3/CD28 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Phosphorylated Y402 is activating and plays a role in activation of Jnk1 and p38 Map kinase.

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
anti-CD3/CD28 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Phosphorylated Y402 is activating and plays a role in activation of Jnk1 and p38 Map kinase.

T180-p - P38A (human)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
anti-CD3/CD28 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Phosphorylated Y402 is activating and plays a role in activation of Jnk1 and p38 Map kinase.

Y182-p - P38A (human)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
anti-CD3/CD28 Pyk2 (human) increase increase in mutants Y881F (Grb2 binding site) and Y402F autophosphorylation site
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Phosphorylated Y402 is activating and plays a role in activation of Jnk1 and p38 Map kinase.

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Phosphorylated Y402 is activating and plays a role in activation of Jnk1 and p38 Map kinase.

Y580-p - Pyk2 (human)
Modsite: yIEDEDyykAsVTRL SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y580‑p, Pyk2 iso2 (human): Y580‑p, Pyk2 (mouse): Y580‑p, Pyk2 (rat): Y580‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y881-p - Pyk2 (human)
Modsite: DRTDDLVyLNVMELV SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y881‑p, Pyk2 iso2 (human): Y839‑p, Pyk2 (mouse): Y881‑p, Pyk2 (rat): Y881‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human