Curated Information
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Home > Curated Information Page > PubMed Id: 26550600
Bauer M, et al. (2015) Assessment of current mass spectrometric workflows for the quantification of low abundant proteins and phosphorylation sites. Data Brief 5, 297-304 26550600
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S16-p - MAD1L1 (human)
Modsite: MVLstLRsLNNFIsQ SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S16‑p, MAD1L1 (mouse): S16‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S214-p - MAD1L1 (human)
Modsite: kIQELQAsQEARADH SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S214‑p, MAD1L1 (mouse): S214‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S233-p - MAD1L1 (human)
Modsite: KDLEQkLsLQEQDAA SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S233‑p, MAD1L1 (mouse): C233‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S428-p - MAD1L1 (human)
Modsite: ELtPAEYsPQLtRRM SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S428‑p, MAD1L1 (mouse): S428‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thymidine increase
nocodazole increase
MG132 increase

S484-p - MAD1L1 (human)
Modsite: LkMLkSQsssAEQsF SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S484‑p, MAD1L1 (mouse): T484‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S485-p - MAD1L1 (human)
Modsite: kMLkSQsssAEQsFL SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S485‑p, MAD1L1 (mouse): S485‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S486-p - MAD1L1 (human)
Modsite: MLkSQsssAEQsFLF SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S486‑p, MAD1L1 (mouse): S486‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S490-p - MAD1L1 (human)
Modsite: QsssAEQsFLFsREE SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S490‑p, MAD1L1 (mouse): S490‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S494-p - MAD1L1 (human)
Modsite: AEQsFLFsREEADtL SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S494‑p, MAD1L1 (mouse): C494‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

T500-p - MAD1L1 (human)
Modsite: FsREEADtLRLkVEE SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T500‑p, MAD1L1 (mouse): A500‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S513-p - MAD1L1 (human)
Modsite: EELEGERsRLEEEKR SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S513‑p, MAD1L1 (mouse): S513‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S538-p - MAD1L1 (human)
Modsite: LQGDyDQsRTKVLHM SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S538‑p, MAD1L1 (mouse): S538‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S562-p - MAD1L1 (human)
Modsite: QRLREDHsQLQAECE SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S562‑p, MAD1L1 (mouse): D562‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human