Curated Information
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Home > Curated Information Page > PubMed Id: 26477307
Yu EJ, et al. (2016) Positive regulation of β-catenin-PROX1 signaling axis by DBC1 in colon cancer progression. Oncogene 35, 3410-8 26477307
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K49-ac - CTNNB1 (human)
Modsite: TAPsLsGkGNPEEED SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): K49‑ac, CTNNB1 (mouse): K49‑ac, CTNNB1 (rat): K49‑ac, CTNNB1 (rabbit): K49‑ac
Characterization
Methods used to characterize site in vivo modification-specific antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  E.coli (bacterial), HEK293T (epithelial), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  bacteria, human
Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) co-immunoprecipitation, transfection of wild-type enzyme
DEACETYLASE SIRT1 (human) co-immunoprecipitation, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DBC1 (human) increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LEF-1 (human) Induces in vitro, co-immunoprecipitation

K345-ac - CTNNB1 (human)
Modsite: WTTSRVLkVLSVCSS SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): K345‑ac, CTNNB1 (mouse): K345‑ac, CTNNB1 (rat): K345‑ac, CTNNB1 (rabbit): K345‑ac
Characterization
Methods used to characterize site in vivo modification-specific antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  E.coli (bacterial), HEK293T (epithelial), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  bacteria, human
Enzymes shown to modify site in vitro
Type Enzyme
DEACETYLASE SIRT1 (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
LEF-1 (human) Induces in vitro, co-immunoprecipitation