Curated Information
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Home > Curated Information Page > PubMed Id: 26507661
Nagy Z, et al. (2015) Cyclic Nucleotide-dependent Protein Kinases Target ARHGAP17 and ARHGEF6 Complexes in Platelets. J Biol Chem 290, 29974-83 26507661
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S702-p - ARHGAP17 (human)
Modsite: LsAPRRYsssLsPIQ SwissProt Entrez-Gene
Orthologous residues
ARHGAP17 (human): S702‑p, ARHGAP17 iso2 (human): S624‑p, ARHGAP17 iso5 (human): S702‑p, ARHGAP17 (mouse): S698‑p, ARHGAP17 iso2 (mouse): S620‑p, ARHGAP17 (rat): S710‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), platelet-blood
Cellular systems studied:  cell lines, tissue
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
colforsin increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FNBP1L (human) Induces co-immunoprecipitation
TRIP10 (human) Disrupts co-immunoprecipitation

S684-p - ARHGEF6 (human)
Modsite: GSSTRkDsIPQVLLP SwissProt Entrez-Gene
Orthologous residues
ARHGEF6 (human): S684‑p, ARHGEF6 iso2 (human): S530‑p, ARHGEF6 (mouse): S679‑p, ARHGEF6 (rat): S680‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), platelet-blood
Cellular systems studied:  cell lines, tissue
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKG1 (human) electrophoretic mobility shift
KINASE PKACA (human) electrophoretic mobility shift
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
colforsin increase