Curated Information
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Home > Curated Information Page > PubMed Id: 26440524
Li L, et al. (2015) Scutellarin's Cardiovascular Endothelium Protective Mechanism: Important Role of PKG-Iα. PLoS One 10, e0139570 26440524
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S239-p - VASP (human)
Modsite: GAKLRKVsKQEEASG SwissProt Entrez-Gene
Orthologous residues
VASP (human): S239‑p, VASP (mouse): S235‑p, VASP (rat): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
scutellarin increase

S45-p - PKG1 (rat)
Modsite: RKLHKCQsVLPVPsT SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): S52‑p, PKG1 (mouse): S45‑p, PKG1 iso2 (mouse): , PKG1 (rat): S45‑p, PKG1 (cow): S45‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia/reoxygenation, scutellarin augment treatment-induced increase

S51-p - PKG1 (rat)
Modsite: QsVLPVPsTHIGPRT SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): S67‑p, PKG1 (mouse): S51‑p, PKG1 iso2 (mouse): , PKG1 (rat): S51‑p, PKG1 (cow): S51‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia/reoxygenation decrease
hypoxia/reoxygenation, scutellarin augment treatment-induced increase

T59-p - PKG1 (rat)
Modsite: THIGPRTtRAQGIsA SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): T75‑p, PKG1 (mouse): T59‑p, PKG1 iso2 (mouse): , PKG1 (rat): T59‑p, PKG1 (cow): T59‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia/reoxygenation, scutellarin augment treatment-induced increase

S65-p - PKG1 (rat)
Modsite: TtRAQGIsAEPQtYR SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): S81‑p, PKG1 (mouse): S65‑p, PKG1 iso2 (mouse): , PKG1 (rat): S65‑p, PKG1 (cow): S65‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)

T70-p - PKG1 (rat)
Modsite: GIsAEPQtYRsFHDL SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): A86‑p, PKG1 (mouse): T70‑p, PKG1 iso2 (mouse): , PKG1 (rat): T70‑p, PKG1 (cow): T70‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)

S73-p - PKG1 (rat)
Modsite: AEPQtYRsFHDLRQA SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): D88‑p, PKG1 (mouse): S73‑p, PKG1 iso2 (mouse): , PKG1 (rat): S73‑p, PKG1 (cow): S73‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia/reoxygenation decrease
hypoxia/reoxygenation, scutellarin augment treatment-induced increase

S90-p - PKG1 (rat)
Modsite: KFTKSERsKDLIKEA SwissProt Entrez-Gene
Orthologous residues
PKG1 (human): , PKG1 iso2 (human): S106‑p, PKG1 (mouse): S90‑p, PKG1 iso2 (mouse): , PKG1 (rat): S90‑p, PKG1 (cow): S90‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia/reoxygenation decrease
hypoxia/reoxygenation, scutellarin augment treatment-induced increase

S236-p - VASP (rat)
Modsite: GAKLRKVsKEEASGG SwissProt Entrez-Gene
Orthologous residues
VASP (human): S239‑p, VASP (mouse): S235‑p, VASP (rat): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, microvessel endothelial
Cellular systems studied:  tissue
Species studied:  human, rat
Comments:  human cardiac microvascular endothelial cells (HCMEC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypoxia/reoxygenation decrease
ischemia/reperfusion decrease