Stretton C, et al. (2015) GSK3-mediated raptor phosphorylation supports amino-acid-dependent mTORC1-directed signalling. Biochem J 470, 207-21 26348909

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T37-p - 4E-BP1 (human) ▲
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p

T46-p - 4E-BP1 (human) ▲
Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p

T412-p - p70S6K (human) ▲
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p

S722-p - Raptor (human) ▲

Modsite: PrLrsVssyGNIRAV SwissProt Entrez-Gene Orthologous residues Raptor (human): S722‑p, Raptor (mouse): S722‑p, Raptor (rat): S722‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Comments:  S863 priming site for S859

S792-p - Raptor (human) ▲

Modsite: DKMRRAssYSsLNSL SwissProt Entrez-Gene Orthologous residues Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Comments:  S863 priming site for S859

S859-p - Raptor (human) ▲

Modsite: DtssLtQsAPAsPtN SwissProt Entrez-Gene Orthologous residues Raptor (human): S859‑p, Raptor (mouse): S859‑p, Raptor (rat): S859‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Comments:  S863 priming site for S859 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes amino_acid_starvation decrease amino_acids amino_acid_starvation inhibit treatment-induced decrease rapamycin amino_acids no effect upon treatment-induced increase KU-0063794 amino_acids no effect upon treatment-induced increase SB415286 decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays mTOR (human) Induces co-immunoprecipitation

S863-p - Raptor (human) ▲

Modsite: LtQsAPAsPtNkGVH SwissProt Entrez-Gene Orthologous residues Raptor (human): S863‑p, Raptor (mouse): S863‑p, Raptor (rat): S863‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Comments:  S863 priming site for S859 Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes amino_acid_starvation decrease amino_acids amino_acid_starvation inhibit treatment-induced decrease rapamycin amino_acids no effect upon treatment-induced increase KU-0063794 amino_acids no effect upon treatment-induced increase

S877-p - Raptor (human) ▲

Modsite: HIHQAGGsPPAssts SwissProt Entrez-Gene Orthologous residues Raptor (human): S877‑p, Raptor (mouse): S877‑p, Raptor (rat): S877‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Comments:  S863 priming site for S859

S240-p - S6 (human) ▲
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene Orthologous residues S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p

S244-p - S6 (human) ▲
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene Orthologous residues S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p

S758-p - ULK1 (human) ▲
Modsite: PVVFtVGsPPsGStP SwissProt Entrez-Gene Orthologous residues ULK1 (human): S758‑p, ULK1 (mouse): S757‑p, ULK1 (rat): S757‑p Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SB415286 decrease