Curated Information
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Home > Curated Information Page > PubMed Id: 26348909
Stretton C, et al. (2015) GSK3-mediated raptor phosphorylation supports amino-acid-dependent mTORC1-directed signalling. Biochem J 470, 207-21 26348909
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T37-p - 4E-BP1 (human)
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human

T46-p - 4E-BP1 (human)
Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human

S722-p - Raptor (human)
Modsite: PrLrsVssyGNIRAV SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S722‑p, Raptor (mouse): S722‑p, Raptor (rat): S722‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Comments:  S863 priming site for S859

S792-p - Raptor (human)
Modsite: DKMRRASsYSsLNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Comments:  S863 priming site for S859

S859-p - Raptor (human)
Modsite: DtssLtQsAPAsPtN SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S859‑p, Raptor (mouse): S859‑p, Raptor (rat): S859‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Comments:  S863 priming site for S859
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation decrease
amino_acids amino_acid_starvation inhibit treatment-induced decrease
rapamycin amino_acids no effect upon treatment-induced increase
KU-0063794 amino_acids no effect upon treatment-induced increase
SB415286 decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
mTOR (human) Induces co-immunoprecipitation

S863-p - Raptor (human)
Modsite: LtQsAPAsPtNkGVH SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S863‑p, Raptor (mouse): S863‑p, Raptor (rat): S863‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Comments:  S863 priming site for S859
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation decrease
amino_acids amino_acid_starvation inhibit treatment-induced decrease
rapamycin amino_acids no effect upon treatment-induced increase
KU-0063794 amino_acids no effect upon treatment-induced increase

S877-p - Raptor (human)
Modsite: HIHQAGGsPPAssts SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S877‑p, Raptor (mouse): S877‑p, Raptor (rat): S877‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Comments:  S863 priming site for S859

S240-p - S6 (human)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human

S244-p - S6 (human)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human

S758-p - ULK1 (human)
Modsite: PVVFtVGsPPsGStP SwissProt Entrez-Gene
Orthologous residues
ULK1 (human): S758‑p, ULK1 (mouse): S757‑p, ULK1 (rat): S757‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SB415286 decrease