Curated Information
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Home > Curated Information Page > PubMed Id: 26344760
Ogawa T, Hirokawa N (2015) Microtubule Destabilizer KIF2A Undergoes Distinct Site-Specific Phosphorylation Cascades that Differentially Affect Neuronal Morphogenesis. Cell Rep 12, 1774-88 26344760
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T186-p - KIF2A iso1 (mouse)
Modsite: SLDYRPLttADPIDE SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T214‑p, KIF2A iso2 (human): T168‑p, KIF2A (mouse): T213‑p, KIF2A iso1 (mouse): T186‑p, KIF2A iso2 (mouse): T167‑p, KIF2A (rat): T213‑p, KIF2A iso2 (rat): T213‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PAK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase

T187-p - KIF2A iso1 (mouse)
Modsite: LDYRPLttADPIDEH SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T215‑p, KIF2A iso2 (human): T169‑p, KIF2A (mouse): T214‑p, KIF2A iso1 (mouse): T187‑p, KIF2A iso2 (mouse): T168‑p, KIF2A (rat): T214‑p, KIF2A iso2 (rat): T214‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PAK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase

T219-p - KIF2A iso1 (mouse)
Modsite: MKDLDVItIPsKDVV SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T247‑p, KIF2A iso2 (human): T201‑p, KIF2A (mouse): T246‑p, KIF2A iso1 (mouse): T219‑p, KIF2A iso2 (mouse): T200‑p, KIF2A (rat): T246‑p, KIF2A iso2 (rat): T246‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

S222-p - KIF2A iso1 (mouse)
Modsite: LDVItIPsKDVVMVH SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S250‑p, KIF2A iso2 (human): S204‑p, KIF2A (mouse): S249‑p, KIF2A iso1 (mouse): S222‑p, KIF2A iso2 (mouse): S203‑p, KIF2A (rat): S249‑p, KIF2A iso2 (rat): S249‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

T245-p - KIF2A iso1 (mouse)
Modsite: TRYLENQtFRFDYAF SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T273‑p, KIF2A iso2 (human): T227‑p, KIF2A (mouse): T272‑p, KIF2A iso1 (mouse): T245‑p, KIF2A iso2 (mouse): T226‑p, KIF2A (rat): T272‑p, KIF2A iso2 (rat): T272‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MLK3 (human)

S255-p - KIF2A iso1 (mouse)
Modsite: FDYAFDDsAPNEMVY SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S283‑p, KIF2A iso2 (human): S237‑p, KIF2A (mouse): S282‑p, KIF2A iso1 (mouse): S255‑p, KIF2A iso2 (mouse): S236‑p, KIF2A (rat): S282‑p, KIF2A iso2 (rat): S282‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

S300-p - KIF2A iso1 (mouse)
Modsite: HTMGGDFsGKNQDCS SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S328‑p, KIF2A iso2 (human): S282‑p, KIF2A (mouse): S327‑p, KIF2A iso1 (mouse): S300‑p, KIF2A iso2 (mouse): S281‑p, KIF2A (rat): S327‑p, KIF2A iso2 (rat): S327‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

T439-p - KIF2A iso1 (mouse)
Modsite: GNERGADtssADRQT SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T467‑p, KIF2A iso2 (human): T421‑p, KIF2A (mouse): T466‑p, KIF2A iso1 (mouse): T439‑p, KIF2A iso2 (mouse): T420‑p, KIF2A (rat): T466‑p, KIF2A iso2 (rat): T466‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

S440-p - KIF2A iso1 (mouse)
Modsite: NERGADtssADRQTR SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S468‑p, KIF2A iso2 (human): S422‑p, KIF2A (mouse): S467‑p, KIF2A iso1 (mouse): S440‑p, KIF2A iso2 (mouse): S421‑p, KIF2A (rat): S467‑p, KIF2A iso2 (rat): S467‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

S441-p - KIF2A iso1 (mouse)
Modsite: ERGADtssADRQTRL SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S469‑p, KIF2A iso2 (human): S423‑p, KIF2A (mouse): S468‑p, KIF2A iso1 (mouse): S441‑p, KIF2A iso2 (mouse): S422‑p, KIF2A (rat): S468‑p, KIF2A iso2 (rat): S468‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse

S456-p - KIF2A iso1 (mouse)
Modsite: EGAEINKsLLALKEC SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S484‑p, KIF2A iso2 (human): S438‑p, KIF2A (mouse): S483‑p, KIF2A iso1 (mouse): S456‑p, KIF2A iso2 (mouse): S437‑p, KIF2A (rat): S483‑p, KIF2A iso2 (rat): S483‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (human)

T474-p - KIF2A iso1 (mouse)
Modsite: LGRNKPHtPFRAsKL SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T502‑p, KIF2A iso2 (human): T456‑p, KIF2A (mouse): T501‑p, KIF2A iso1 (mouse): T474‑p, KIF2A iso2 (mouse): T455‑p, KIF2A (rat): T501‑p, KIF2A iso2 (rat): T501‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase

S479-p - KIF2A iso1 (mouse)
Modsite: PHtPFRAsKLtQVLR SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S507‑p, KIF2A iso2 (human): S461‑p, KIF2A (mouse): S506‑p, KIF2A iso1 (mouse): S479‑p, KIF2A iso2 (mouse): S460‑p, KIF2A (rat): S506‑p, KIF2A iso2 (rat): S506‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PAK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase

T482-p - KIF2A iso1 (mouse)
Modsite: PFRAsKLtQVLRDsF SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): T510‑p, KIF2A iso2 (human): T464‑p, KIF2A (mouse): T509‑p, KIF2A iso1 (mouse): T482‑p, KIF2A iso2 (mouse): T463‑p, KIF2A (rat): T509‑p, KIF2A iso2 (rat): T509‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ROCK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPA increase

S488-p - KIF2A iso1 (mouse)
Modsite: LtQVLRDsFIGENSR SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S516‑p, KIF2A iso2 (human): S470‑p, KIF2A (mouse): S515‑p, KIF2A iso1 (mouse): S488‑p, KIF2A iso2 (mouse): S469‑p, KIF2A (rat): S515‑p, KIF2A iso2 (rat): S515‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PAK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
BDNF increase

S503-p - KIF2A iso1 (mouse)
Modsite: TCMIATIsPGMASCE SwissProt Entrez-Gene
Orthologous residues
KIF2A (human): S531‑p, KIF2A iso2 (human): S485‑p, KIF2A (mouse): S530‑p, KIF2A iso1 (mouse): S503‑p, KIF2A iso2 (mouse): S484‑p, KIF2A (rat): S530‑p, KIF2A iso2 (rat): S530‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical', COS (fibroblast)
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)