Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18951975
Wei Z, et al. (2009) Haloperidol disrupts Akt signalling to reveal a phosphorylation-dependent regulation of pro-apoptotic Bcl-XS function. Cell Signal 21, 161-8 18951975
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial), neuron, PC-12 (chromaffin)
Cellular systems studied:  cell lines, primary cells
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
haloperidol decrease

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial), neuron, PC-12 (chromaffin)
Cellular systems studied:  cell lines, primary cells
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
haloperidol decrease
DTG no change compared to control
LY294002 decrease

S137-p - BAD (rat)
Modsite: PFRGRsRsAPPNLWA SwissProt Entrez-Gene
Orthologous residues
BAD (human): S99‑p, BAD (mouse): S136‑p, BAD (rat): S137‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial), neuron, PC-12 (chromaffin)
Cellular systems studied:  cell lines, primary cells
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
haloperidol decrease

S62-p - Bcl-xL (rat)
Modsite: PSWHLADsPAVNGAT SwissProt Entrez-Gene
Orthologous residues
Bcl‑xL (human): S62‑p, Bcl‑xL (mouse): S62‑p, Bcl‑xL (rat): S62‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial), neuron, PC-12 (chromaffin)
Cellular systems studied:  cell lines, primary cells
Species studied:  human, rat

S106-p - Bcl-xL (rat)
Modsite: LRYRRAFsDLTSQLH SwissProt Entrez-Gene
Orthologous residues
Bcl‑xL (human): S106‑p, Bcl‑xL (mouse): S106‑p, Bcl‑xL (rat): S106‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial), neuron, PC-12 (chromaffin)
Cellular systems studied:  cell lines, primary cells
Species studied:  human, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) co-immunoprecipitation, phospho-motif antibody, modification site within consensus motif, transfection of constitutively active enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  apoptosis, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
VDAC1 (rat) Disrupts co-immunoprecipitation
Akt1 (rat) Induces co-immunoprecipitation

S228-p - Bcl-xL (rat)
Modsite: AGVVLLGsLFSRK__ SwissProt Entrez-Gene
Orthologous residues
Bcl‑xL (human): S228‑p, Bcl‑xL (mouse): S228‑p, Bcl‑xL (rat): S228‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  adrenal cancer, pheochromocytoma
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial), neuron, PC-12 (chromaffin)
Cellular systems studied:  cell lines, primary cells
Species studied:  human, rat