Curated Information
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Home > Curated Information Page > PubMed Id: 11684675
Gonzalez-Garcia A, et al. (2002) A new role for the p85-phosphatidylinositol 3-kinase regulatory subunit linking FRAP to p70 S6 kinase activation. J Biol Chem 277, 1500-8 11684675
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T252-p - p70S6K (human)
Modsite: HDGtVtHtFCGtIEY SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
Methods used to characterize site in vivo phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PIK3CA (human) transfection of dominant-negative enzyme, transfection of constitutively active enzyme, phosphopeptide analysis, 2D analysis P65 PI3K acts as a dominant negative repressor of PI3K activity
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PIK3CA (human) transfection of dominant-negative enzyme, transfection of constitutively active enzyme, phosphopeptide analysis, 2D analysis P65 PI3K acts as a dominant negative repressor of PI3K activity
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S434-p - p70S6K (human)
Modsite: sFEPKIRsPRRFIGs SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S434‑p, p70S6K iso2 (human): S411‑p, p70S6K (mouse): S434‑p, p70S6K (rat): S434‑p, p70S6K iso2 (rat): S411‑p, p70S6K (fruit fly): S418‑p
Characterization
Methods used to characterize site in vivo phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey

S441-p - p70S6K (human)
Modsite: sPRRFIGsPRtPVsP SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S441‑p, p70S6K iso2 (human): S418‑p, p70S6K (mouse): S441‑p, p70S6K (rat): S441‑p, p70S6K iso2 (rat): S418‑p, p70S6K (fruit fly): Q425‑p
Characterization
Methods used to characterize site in vivo phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey

S447-p - p70S6K (human)
Modsite: GsPRtPVsPVkFsPG SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): S430‑p
Characterization
Methods used to characterize site in vivo phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey