Curated Information
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Home > Curated Information Page > PubMed Id: 14555661
Knights CD, Liu Y, Appella E, Kulesz-Martin M (2003) Defective p53 post-translational modification required for wild type p53 inactivation in malignant epithelial cells with mdm2 gene amplification. J Biol Chem 278, 52890-900 14555661
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S18-p - p53 (mouse)
Modsite: IsLELPLsQEtFsGL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  epidermal
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase

T21-p - p53 (mouse)
Modsite: ELPLsQEtFsGLWKL SwissProt Entrez-Gene
Orthologous residues
p53 (human): T18‑p, p53 iso2 (human): T18‑p, p53 iso4 (human): , p53 (mouse): T21‑p, p53 iso2 (mouse): T21‑p, p53 (rat): T18‑p, p53 (rabbit): T18‑p, p53 (green monkey): T18‑p
Characterization
Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  epidermal
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MDM2 (mouse) Disrupts co-immunoprecipitation
p300 (mouse) Induces co-immunoprecipitation

S23-p - p53 (mouse)
Modsite: PLsQEtFsGLWKLLP SwissProt Entrez-Gene
Orthologous residues
p53 (human): S20‑p, p53 iso2 (human): S20‑p, p53 iso4 (human): , p53 (mouse): S23‑p, p53 iso2 (mouse): S23‑p, p53 (rat): S20‑p, p53 (rabbit): S20‑p, p53 (green monkey): S20‑p
Characterization
Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  epidermal
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase

S37-p - p53 (mouse)
Modsite: PPEDILPsPHCMDDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): L35‑p, p53 iso2 (human): L35‑p, p53 iso4 (human): , p53 (mouse): S37‑p, p53 iso2 (mouse): S37‑p, p53 (rat): T35‑p, p53 (rabbit): T33‑p, p53 (green monkey): L35‑p
Characterization
Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  epidermal
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase

K379-ac - p53 (mouse)
Modsite: QsTsRHkkTMVkkVG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K382‑ac, p53 iso2 (human): , p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): , p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac
Characterization
Methods used to characterize site in vivo modification-specific antibody, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  epidermal
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
UV increase