Curated Information
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Home > Curated Information Page > PubMed Id: 18922800
Chi YH, et al. (2008) Requirements for protein phosphorylation and the kinase activity of polo-like kinase 1 (Plk1) for the kinetochore function of mitotic arrest deficiency protein 1 (Mad1). J Biol Chem 283, 35834-44 18922800
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S22-p - MAD1L1 (human)
Modsite: RsLNNFIsQRVEGGs SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S22‑p, MAD1L1 (mouse): S22‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) transfection of wild-type enzyme, co-immunoprecipitation

S29-p - MAD1L1 (human)
Modsite: sQRVEGGsGLDISTS SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S29‑p, MAD1L1 (mouse): S29‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) transfection of wild-type enzyme, co-immunoprecipitation

S428-p - MAD1L1 (human)
Modsite: ELtPAEYsPQLtRRM SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S428‑p, MAD1L1 (mouse): S428‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

T680-p - MAD1L1 (human)
Modsite: SKMQLLEtEFSHTVG SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T680‑p, MAD1L1 (mouse): T679‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  cell cycle regulation

S699-p - MAD1L1 (human)
Modsite: VHLRRQDsIPAFLSS SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S699‑p, MAD1L1 (mouse): S698‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

T708-p - MAD1L1 (human)
Modsite: PAFLSSLtLELFSRQ SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T708‑p, MAD1L1 (mouse): T707‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  intracellular localization