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Home > Curated Information Page > PubMed Id: 15743786
Motti ML, et al. (2005) Complex regulation of the cyclin-dependent kinase inhibitor p27kip1 in thyroid cancer cells by the PI3K/AKT pathway: regulation of p27kip1 expression and localization. Am J Pathol 166, 737-49 15743786
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  thyroid cancer, anaplastic thyroid carcinoma, follicular thyroid carcinoma, papillary thyroid carcinoma
Relevant cell lines - cell types - tissues:  thyroid, TPC-1 (thyroid cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  NPA, WRO, FRO, FB-1 cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
Associated Diseases
Diseases Alterations Comments
follicular thyroid carcinoma, papillary thyroid carcinoma, anaplastic thyroid carcinoma increased

S10-p - p27Kip1 (human)
Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  thyroid cancer, anaplastic thyroid carcinoma, follicular thyroid carcinoma, papillary thyroid carcinoma
Relevant cell lines - cell types - tissues:  thyroid, TPC-1 (thyroid cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  NPA, WRO, FRO, FB-1 cells

T157-p - p27Kip1 (human)
Modsite: GIRkrPAtDDSSTQN SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T157‑p, p27Kip1 (mouse): A157‑p, p27Kip1 (rat): A157‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  thyroid cancer, anaplastic thyroid carcinoma, follicular thyroid carcinoma, papillary thyroid carcinoma
Relevant cell lines - cell types - tissues:  thyroid, TPC-1 (thyroid cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  NPA, WRO, FRO, FB-1 cells
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, phospho-antibody, genetic transfer of constitutively active upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization
Associated Diseases
Diseases Alterations Comments
follicular thyroid carcinoma, papillary thyroid carcinoma, anaplastic thyroid carcinoma increased

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  thyroid cancer, anaplastic thyroid carcinoma, follicular thyroid carcinoma, papillary thyroid carcinoma
Relevant cell lines - cell types - tissues:  thyroid, TPC-1 (thyroid cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Comments:  NPA, WRO, FRO, FB-1 cells
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) pharmacological inhibitor of upstream enzyme, phospho-antibody, genetic transfer of constitutively active upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LY294002 no change compared to control
LY294002 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization
Associated Diseases
Diseases Alterations Comments
follicular thyroid carcinoma, papillary thyroid carcinoma, anaplastic thyroid carcinoma increased