Ge X, et al. (2009) PCAF acetylates {beta}-catenin and improves its stability. Mol Biol Cell 20, 419-27 18987336

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K19-ac - CTNNB1 (human) ▲

Modsite: MAMEPDRkAAVsHWQ SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K19‑ac, CTNNB1 (mouse): K19‑ac, CTNNB1 (rat): K19‑ac, CTNNB1 (rabbit): K19‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE PCAF (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) transfection of wild-type enzyme, transfection of inactive enzyme, co-immunoprecipitation, siRNA inhibition of enzyme, microscopy-colocalization Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Effect of modification (process):  carcinogenesis, inhibited, cell motility, induced, transcription, induced

K49-ac - CTNNB1 (human) ▲

Modsite: TAPsLsGkGNPEEED SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): K49‑ac, CTNNB1 (mouse): K49‑ac, CTNNB1 (rat): K49‑ac, CTNNB1 (rabbit): K49‑ac Characterization Methods used to characterize site in vivo:  [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme ACETYLTRANSFERASE PCAF (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes ACETYLTRANSFERASE PCAF (human) transfection of wild-type enzyme, transfection of inactive enzyme, co-immunoprecipitation, siRNA inhibition of enzyme, microscopy-colocalization Downstream Regulation Effect of modification (function):  intracellular localization, protein stabilization Effect of modification (process):  carcinogenesis, inhibited, cell motility, induced, transcription, induced