Curated Information
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Home > Curated Information Page > PubMed Id: 18987336
Ge X, et al. (2009) PCAF acetylates {beta}-catenin and improves its stability. Mol Biol Cell 20, 419-27 18987336
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K19-ac - CTNNB1 (human)
Modsite: MAMEPDRkAAVsHWQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): K19‑ac, CTNNB1 (mouse): K19‑ac, CTNNB1 (rat): K19‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE PCAF (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE PCAF (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, microscopy-colocalization, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Effect of modification (process):  carcinogenesis, inhibited, cell motility, induced, transcription, induced

K49-ac - CTNNB1 (human)
Modsite: TAPsLsGkGNPEEED SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): K49‑ac, CTNNB1 (mouse): K49‑ac, CTNNB1 (rat): K49‑ac
Characterization
Methods used to characterize site in vivo [14C] acetate labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE PCAF (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE PCAF (human) siRNA inhibition of enzyme, co-immunoprecipitation, transfection of wild-type enzyme, microscopy-colocalization, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein stabilization
Effect of modification (process):  carcinogenesis, inhibited, cell motility, induced, transcription, induced