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Home > Curated Information Page > PubMed Id: 15306821
Um SH, et al. (2004) Absence of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity. Nature 431, 200-5 15306821
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA S6 (human) no change compared to control
insulin increase
insulin siRNA S6 (human) augment treatment-induced increase

S312-p - IRS1 (human)
Modsite: tEsItAtsPAsMVGG SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
siRNA insulin p70S6K (human) inhibit treatment-induced increase

S636-p - IRS1 (human)
Modsite: sGDyMPMsPKsVSAP SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S636‑p, IRS1 (mouse): S632‑p, IRS1 (rat): S632‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
siRNA insulin p70S6K (human) inhibit treatment-induced increase

S639-p - IRS1 (human)
Modsite: yMPMsPKsVSAPQQI SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S639‑p, IRS1 (mouse): S635‑p, IRS1 (rat): S635‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
siRNA insulin p70S6K (human) inhibit treatment-induced increase

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
siRNA insulin p70S6K (human) inhibit treatment-induced increase

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase WT and S6 -/- fat, liver, muscle
high-fat diet insulin inhibit treatment-induced increase WT, fat, liver, muscle
high-fat diet insulin no effect upon treatment-induced increase S6 -/- fat, liver, muscle
no change compared to control normal diet WT, K/K A and ob/ob mice

S307-p - IRS1 (mouse)
Modsite: tEsITAtsPAsMVGG SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase WT
high-fat diet no change compared to control S6 -/-
increase normal diet WT, K/K A and ob/ob mice

S632-p - IRS1 (mouse)
Modsite: NGDyMPMsPKsVsAP SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S636‑p, IRS1 (mouse): S632‑p, IRS1 (rat): S632‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase WT
high-fat diet no change compared to control S6 -/-
increase normal diet WT, K/K A and ob/ob mice

S635-p - IRS1 (mouse)
Modsite: yMPMsPKsVsAPQQI SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S639‑p, IRS1 (mouse): S635‑p, IRS1 (rat): S635‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase WT
high-fat diet no change compared to control S6 -/-
increase normal diet WT, K/K A and ob/ob mice

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase S6 -/-
increase normal diet, K/K A, ob/ob- obese mouse models
no change compared to control WT normal diet, K/K A, ob/ob- obese mouse models
insulin no change compared to control muscle, WT
high-fat diet no change compared to control muscle, WT
high-fat diet insulin increase muscle, WT
insulin increase fat tissue, WT
high-fat diet increase fat tissue, WT
high-fat diet insulin augment treatment-induced increase fat tissue, WT

S240-p - S6 (mouse)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase S6 -/-
increase normal diet, K/K A, ob/ob- obese mouse models
no change compared to control WT normal diet, K/K A, ob/ob- obese mouse models

S244-p - S6 (mouse)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Relevant cell lines - cell types - tissues:  adipose tissue [p70S6K (mouse), homozygous knockout], HeLa (cervical), liver [p70S6K (mouse), homozygous knockout], muscle [p70S6K (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase S6 -/-
increase normal diet, K/K A, ob/ob- obese mouse models
no change compared to control WT normal diet, K/K A, ob/ob- obese mouse models