Curated Information
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Home > Curated Information Page > PubMed Id: 12384459
Boo YC, et al. (2002) Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism. Am J Physiol Heart Circ Physiol 283, H1819-28 12384459
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T308-p - Akt1 (cow)
Modsite: KDGATMKtFCGTPEY SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid_shear_stress increase
wortmannin fluid_shear_stress inhibit treatment-induced increase
LY294002 fluid_shear_stress inhibit treatment-induced increase
mPKI fluid_shear_stress no effect upon treatment-induced increase
H-89 fluid_shear_stress augment treatment-induced increase
VEGF increase
cAMP_analog increase
wortmannin cAMP_analog inhibit treatment-induced increase

S473-p - Akt1 (cow)
Modsite: RPHFPQFsYSASATA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid_shear_stress increase
VEGF increase
cAMP_analog increase

S116-p - eNOS (cow)
Modsite: RKLQTRPsPGPPPAE SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S114‑p, eNOS (mouse): T113‑p, eNOS (rat): T113‑p, eNOS (rabbit): T120‑p, eNOS (pig): S116‑p, eNOS (cow): S116‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid_shear_stress no change compared to control
VEGF no change compared to control
cAMP_analog no change compared to control
high_cell_density decrease

T497-p - eNOS (cow)
Modsite: AGITRKktFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid_shear_stress no change compared to control
VEGF no change compared to control
cAMP_analog decrease

S635-p - eNOS (cow)
Modsite: WRRKRKEsSNTDSAG SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid_shear_stress increase
wortmannin fluid_shear_stress no effect upon treatment-induced increase
LY294002 fluid_shear_stress no effect upon treatment-induced increase
mPKI fluid_shear_stress inhibit treatment-induced increase
H-89 fluid_shear_stress inhibit treatment-induced increase
VEGF increase
cAMP_analog increase
wortmannin cAMP_analog no effect upon treatment-induced increase
H-89 cAMP_analog inhibit treatment-induced increase

S1179-p - eNOS (cow)
Modsite: TSRIRtQsFsLQERH SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fluid_shear_stress increase
wortmannin fluid_shear_stress inhibit treatment-induced increase
LY294002 fluid_shear_stress inhibit treatment-induced increase
mPKI fluid_shear_stress inhibit treatment-induced increase
H-89 fluid_shear_stress inhibit treatment-induced increase
VEGF increase
cAMP_analog increase
wortmannin cAMP_analog inhibit treatment-induced increase
H-89 cAMP_analog inhibit treatment-induced increase