Curated Information
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Home > Curated Information Page > PubMed Id: 11756337
Vecchione C, et al. (2002) Leptin effect on endothelial nitric oxide is mediated through Akt-endothelial nitric oxide synthase phosphorylation pathway. Diabetes 51, 168-73 11756337
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  aorta, HAEC (endothelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase
erbstatin leptin inhibit treatment-induced increase
genistein leptin no effect upon treatment-induced increase
wortmannin leptin no effect upon treatment-induced increase
LY294002 leptin no effect upon treatment-induced increase
insulin increase
LY294002 insulin inhibit treatment-induced increase

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  aorta, HAEC (endothelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase
erbstatin leptin inhibit treatment-induced increase
genistein leptin no effect upon treatment-induced increase
wortmannin leptin no effect upon treatment-induced increase
LY294002 leptin no effect upon treatment-induced increase
insulin increase
LY294002 insulin inhibit treatment-induced increase

S1176-p - eNOS (rat)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  aorta, HAEC (endothelial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase