Curated Information
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Home > Curated Information Page > PubMed Id: 15489898
Wei L, Yang Y, Zhang X, Yu Q (2004) Altered regulation of Src upon cell detachment protects human lung adenocarcinoma cells from anoikis. Oncogene 23, 9052-61 15489898
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y397-p - FAK (human)
Modsite: sVsEtDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), Calu-3 (pulmonary), HBE (epithelial), MDCK (epithelial), NCI-H1437 (pulmonary), NCI-H1792 (pulmonary), NCI-H460 (pulmonary), NCI-H522 (pulmonary), SK-LU-1 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  dog, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_detachment decrease

Y420-p - Fyn (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Fyn (human): Y420‑p, Fyn iso2 (human): Y417‑p, Fyn iso3 (human): Y365‑p, Fyn (mouse): Y420‑p, Fyn (rat): Y420‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_detachment no change compared to control

Y397-p - LYN (human)
Modsite: RVIEDNEytAREGAk SwissProt Entrez-Gene
Orthologous residues
LYN (human): Y397‑p, LYN iso2 (human): Y376‑p, LYN (mouse): Y397‑p, LYN iso2 (mouse): Y376‑p, LYN (rat): Y397‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_detachment no change compared to control

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP2 decrease

Y419-p - Src (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), Calu-3 (pulmonary), HBE (epithelial), MDCK (epithelial), NCI-H1437 (pulmonary), NCI-H1792 (pulmonary), NCI-H460 (pulmonary), NCI-H522 (pulmonary), SK-LU-1 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  dog, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PDGFRA (human) activation of upstream enzyme, antisense inhibition of upstream enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_detachment increase
AG17 cell_detachment inhibit treatment-induced increase
PP2 decrease
cell_detachment no change compared to control

Y530-p - Src (human)
Modsite: FTstEPQyQPGENL_ SwissProt Entrez-Gene
Orthologous residues
Src (human): Y530‑p, Src iso2 (human): Y536‑p, Src (mouse): Y535‑p, Src iso2 (mouse): Y529‑p, Src (rat): Y530‑p, Src (chicken): Y527‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), Calu-3 (pulmonary), HBE (epithelial), MDCK (epithelial), NCI-H1437 (pulmonary), NCI-H1792 (pulmonary), NCI-H460 (pulmonary), NCI-H522 (pulmonary), SK-LU-1 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  dog, human

Y426-p - Yes (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Yes (human): Y426‑p, Yes (mouse): Y424‑p, Yes (rat): Y424‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_detachment increase