Curated Information
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Home > Curated Information Page > PubMed Id: 15314172
Castoria G, et al. (2004) Role of atypical protein kinase C in estradiol-triggered G1/S progression of MCF-7 cells. Mol Cell Biol 24, 7643-53 15314172
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol increase

T638-p - PKCA (human)
Modsite: TRGQPVLtPPDQLVI SwissProt Entrez-Gene
Orthologous residues
PKCA (human): T638‑p, PKCA (mouse): T638‑p, PKCA (rat): T638‑p, PKCA (cow): T638‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol no change compared to control

T642-p - PKCB (human)
Modsite: TRQPVELtPtDKLFI SwissProt Entrez-Gene
Orthologous residues
PKCB (human): T642‑p, PKCB iso2 (human): , PKCB (mouse): T642‑p, PKCB iso2 (mouse): , PKCB (rat): T642‑p, PKCB iso2 (rat): , PKCB (cow):
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol no change compared to control

S645-p - PKCD (human)
Modsite: LNEkARLsysDKNLI SwissProt Entrez-Gene
Orthologous residues
PKCD (human): S645‑p, PKCD iso2 (human): S676‑p, PKCD (mouse): S643‑p, PKCD iso2 (mouse): S669‑p, PKCD (rat): S643‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol no change compared to control

T412-p - PKCI (human)
Modsite: RPGDttstFCGtPNy SwissProt Entrez-Gene
Orthologous residues
PKCI (human): T412‑p, PKCI (mouse): T411‑p, PKCI (rat): T412‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol increase
faslodex estradiol inhibit treatment-induced increase

S676-p - PKCT (human)
Modsite: LNEkPRLsFADRALI SwissProt Entrez-Gene
Orthologous residues
PKCT (human): S676‑p, PKCT (mouse): S676‑p, PKCT (rat): S676‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol no change compared to control

T410-p - PKCZ (human)
Modsite: GPGDtTstFCGtPNy SwissProt Entrez-Gene
Orthologous residues
PKCZ (human): T410‑p, PKCZ iso2 (human): T227‑p, PKCZ iso3 (human): T306‑p, PKCZ (mouse): T410‑p, PKCZ (rat): T410‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), MCF-7 (breast cell)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
estradiol increase
faslodex estradiol inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Comments:  phosphorylation leads to recruitment of Ras to a multimolecular complex