Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 2941417
Shackelford DA, Trowbridge IS (1986) Identification of lymphocyte integral membrane proteins as substrates for protein kinase C. Phosphorylation of the interleukin-2 receptor, class I HLA antigens, and T200 glycoprotein. J Biol Chem 261, 8334-41 2941417
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S268-p - IL2RA (human)
Modsite: WQRRQRKsRRtI___ SwissProt Entrez-Gene
Orthologous residues
IL2RA (human): S268‑p, IL2RA (mouse): S264‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  CCRF-CEM (T lymphocyte), HUT-102 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) phosphopeptide analysis, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase

T271-p - IL2RA (human)
Modsite: RQRKsRRtI______ SwissProt Entrez-Gene
Orthologous residues
IL2RA (human): T271‑p, IL2RA (mouse): T267‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  CCRF-CEM (T lymphocyte), HUT-102 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) phosphopeptide analysis, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase