Yamamoto K, Ichijo H, Korsmeyer SJ (1999) BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Mol Cell Biol 19, 8469-78 10567572

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T69-p - Bcl-2 (human) ▲

Modsite: SRDPVARtsPLQtPA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): T69‑p, Bcl‑2 (mouse): T69‑p, Bcl‑2 (rat): T69‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), WEHI-231 (B lymphocyte) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes taxol increase

S70-p - Bcl-2 (human) ▲

Modsite: RDPVARtsPLQtPAA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S70‑p, Bcl‑2 (mouse): S70‑p, Bcl‑2 (rat): S70‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), WEHI-231 (B lymphocyte) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE JNK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE JNK1 (human) transfection of dominant-negative enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes taxol increase Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  apoptosis, induced

S87-p - Bcl-2 (human) ▲

Modsite: AAAGPALsPVPPVVH SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S87‑p, Bcl‑2 (mouse): S84‑p, Bcl‑2 (rat): S84‑p Characterization Methods used to characterize site in vivo:  2D analysis, [32P] bio-synthetic labeling, electrophoretic mobility shift, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte), WEHI-231 (B lymphocyte) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE JNK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE JNK1 (human) activation of upstream enzyme, transfection of wild-type enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes taxol increase Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  apoptosis, induced