Breitschopf K, et al. (2000) Posttranslational modification of Bcl-2 facilitates its proteasome-dependent degradation: molecular characterization of the involved signaling pathway. Mol Cell Biol 20, 1886-96 10669763

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

T56-p - Bcl-2 (human) ▲

Modsite: FSSQPGHtPHPAASR SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): T56‑p, Bcl‑2 (mouse): N56‑p, Bcl‑2 (rat): N56‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), HUVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE ERK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (human) modification site within consensus motif Downstream Regulation Effect of modification (function):  protein stabilization, ubiquitination Effect of modification (process):  apoptosis, induced

T74-p - Bcl-2 (human) ▲

Modsite: ARtsPLQtPAAPGAA SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): T74‑p, Bcl‑2 (mouse): P74‑p, Bcl‑2 (rat): P74‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), HUVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE ERK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (human) modification site within consensus motif Downstream Regulation Effect of modification (function):  protein stabilization, ubiquitination Effect of modification (process):  apoptosis, induced

S87-p - Bcl-2 (human) ▲

Modsite: AAAGPALsPVPPVVH SwissProt Entrez-Gene Orthologous residues Bcl‑2 (human): S87‑p, Bcl‑2 (mouse): S84‑p, Bcl‑2 (rat): S84‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HeLa (cervical), HUVEC (endothelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE ERK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (human) phospho-motif antibody, transfection of wild-type enzyme, modification site within consensus motif, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF decrease PD98059 decrease Downstream Regulation Effect of modification (function):  protein stabilization, ubiquitination Effect of modification (process):  apoptosis, induced