Curated Information
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Home > Curated Information Page > PubMed Id: 10669763
Breitschopf K, et al. (2000) Posttranslational modification of Bcl-2 facilitates its proteasome-dependent degradation: molecular characterization of the involved signaling pathway. Mol Cell Biol 20, 1886-96 10669763
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T56-p - Bcl-2 (human)
Modsite: FSSQPGHtPHPAASR SwissProt Entrez-Gene
Orthologous residues
Bcl‑2 (human): T56‑p, Bcl‑2 (mouse): N56‑p, Bcl‑2 (rat): N56‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) modification site within consensus motif
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination
Effect of modification (process):  apoptosis, induced

T74-p - Bcl-2 (human)
Modsite: ARtsPLQtPAAPGAA SwissProt Entrez-Gene
Orthologous residues
Bcl‑2 (human): T74‑p, Bcl‑2 (mouse): P74‑p, Bcl‑2 (rat): P74‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) modification site within consensus motif
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination
Effect of modification (process):  apoptosis, induced

S87-p - Bcl-2 (human)
Modsite: AAAGPALsPVPPVVH SwissProt Entrez-Gene
Orthologous residues
Bcl‑2 (human): S87‑p, Bcl‑2 (mouse): S84‑p, Bcl‑2 (rat): S84‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), HUVEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK2 (human) modification site within consensus motif, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, phospho-motif antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF decrease
PD98059 decrease
Downstream Regulation
Effect of modification (function):  protein stabilization, ubiquitination
Effect of modification (process):  apoptosis, induced