Curated Information
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Home > Curated Information Page > PubMed Id: 12167717
Scheid MP, Marignani PA, Woodgett JR (2002) Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Mol Cell Biol 22, 6247-60 12167717
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PRKD1 (human) co-immunoprecipitation, modification site within consensus motif, pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, microscopy-colocalization, pharmacological activator of upstream enzyme
Comments:  Phosphorylation of S473 is required for T308 phosphorylation, but colocalization with PKB removes this requirement.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT increase
LY294002 4-HT inhibit treatment-induced increase
PDK1 (human) increase
4-HT PDK1 (human) augment treatment-induced decrease
LY294002 PDK1 (human) inhibit treatment-induced increase

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PRKD1 (human) co-immunoprecipitation, modification site within consensus motif, pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, microscopy-colocalization, pharmacological activator of upstream enzyme
Comments:  Phosphorylation of S473 is required for T308 phosphorylation, but colocalization with PKB removes this requirement.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
4-HT increase
LY294002 4-HT inhibit treatment-induced increase
PDK1 (human) increase
4-HT PDK1 (human) augment treatment-induced decrease
LY294002 PDK1 (human) inhibit treatment-induced increase

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AP21967 Akt1 (cow) increase

T308-p - Akt1 (cow)
Modsite: KDGATMKtFCGTPEY SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AP21967 increase
LY294002 AP21967 inhibit treatment-induced increase
staurosporine AP21967 inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization
Comments:  Translocation to the plasma membrane leads to phosphorylation.

S473-p - Akt1 (cow)
Modsite: RPHFPQFsYSASATA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AP21967 increase
LY294002 AP21967 inhibit treatment-induced increase
staurosporine AP21967 no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization
Comments:  Translocation to the plasma membrane leads to phosphorylation.