Li C, et al. (2008) Essential phosphatases and a phospho-degron are critical for regulation of SRC-3/AIB1 coactivator function and turnover. Mol Cell 31, 835-49 18922467

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S118-p - ER-alpha (human) ▲

Modsite: LHPPPQLsPFLQPHG SwissProt Entrez-Gene Orthologous residues ER‑alpha (human): S118‑p, ER‑alpha (mouse): S122‑p, ER‑alpha (rat): S123‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase

T24-p - SRC-3 (human) ▲

Modsite: KRKLPCDtPGQGLtC SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): T24‑p, SRC‑3 iso2 (human): T24‑p, SRC‑3 (mouse): A25‑p, SRC‑3 (rat): ‑ Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP2CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PPP1CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PDXP (human) transfection of wild-type enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase

S101-p - SRC-3 (human) ▲

Modsite: DVQkADVssTGQGVI SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S101‑p, SRC‑3 iso2 (human): S101‑p, SRC‑3 (mouse): S102‑p, SRC‑3 (rat): ‑ Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme PHOSPHATASE PPP1CA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP1CA (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  protein degradation

S102-p - SRC-3 (human) ▲

Modsite: VQkADVssTGQGVID SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S102‑p, SRC‑3 iso2 (human): S102‑p, SRC‑3 (mouse): S103‑p, SRC‑3 (rat): ‑ Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme PHOSPHATASE PPP1CA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP1CA (human) co-immunoprecipitation Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  cell growth, altered

S505-p - SRC-3 (human) ▲

Modsite: SPVAGVHsPMAsSGN SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S505‑p, SRC‑3 iso2 (human): S505‑p, SRC‑3 (mouse): S498‑p, SRC‑3 (rat): S205‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP1CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PPP2CA (human) siRNA inhibition of enzyme, transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PDXP (human) siRNA inhibition of enzyme, transfection of wild-type enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase

S543-p - SRC-3 (human) ▲

Modsite: SLLSTLSsPGPKLDN SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S543‑p, SRC‑3 iso2 (human): S543‑p, SRC‑3 (mouse): S536‑p, SRC‑3 (rat): S243‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PDXP (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PPP1CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PPP2CA (human) siRNA inhibition of enzyme, transfection of wild-type enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase

S857-p - SRC-3 (human) ▲

Modsite: PPYNRAVsLDsPVsV SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S857‑p, SRC‑3 iso2 (human): S857‑p, SRC‑3 (mouse): S847‑p, SRC‑3 (rat): S551‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP2CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PDXP (human) transfection of wild-type enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol no change compared to control

S860-p - SRC-3 (human) ▲

Modsite: NRAVsLDsPVsVGss SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S860‑p, SRC‑3 iso2 (human): S860‑p, SRC‑3 (mouse): S850‑p, SRC‑3 (rat): S554‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP2CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PDXP (human) transfection of wild-type enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase

S867-p - SRC-3 (human) ▲

Modsite: sPVsVGssPPVKNIS SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S867‑p, SRC‑3 iso2 (human): S867‑p, SRC‑3 (mouse): G857‑p, SRC‑3 (rat): V561‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  breast cancer Relevant cell lines - cell types - tissues:  HEK293T (epithelial), ZR-75-1 (breast cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP2CA (human) transfection of wild-type enzyme, phospho-antibody PHOSPHATASE PDXP (human) transfection of wild-type enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes estradiol increase