Curated Information
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Home > Curated Information Page > PubMed Id: 15574412
Liberman Z, Eldar-Finkelman H (2005) Serine 332 phosphorylation of insulin receptor substrate-1 by glycogen synthase kinase-3 attenuates insulin signaling. J Biol Chem 280, 4422-8 15574412
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast) [INSR (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast) [INSR (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

S332-p - IRS1 (rat)
Modsite: sDGEGTMsRPAsVDG SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S337‑p, IRS1 (mouse): S332‑p, IRS1 (rat): S332‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [INSR (human)]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
insulin no change compared to control
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  decreases tyrosine phosphorylation of IRS-1 and Akt1 phosphorylation upon insulin stimulation

S336-p - IRS1 (rat)
Modsite: GTMsRPAsVDGSPVs SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S341‑p, IRS1 (mouse): S336‑p, IRS1 (rat): S336‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [INSR (human)]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  decreases tyrosine phosphorylation of IRS-1 and Akt1 phosphorylation upon insulin stimulation, primes S332 for phosphorylation by GSK3B